中国蔬菜Issue(3)：45-49,5.
黄瓜棒孢叶斑病病原菌RFP标记转化株的构建
Construction of RFP Transformation as a Marker in Corynespora cassiicola from Cucumber
摘要
Abstract
Cucumber Corynespora leaf spot caused by Corynespora cassiicola has become an important new epidemic disease in cucumber production in China.At present,there is no resistant varieties and the infection mechanism of C. cassiicola and its interaction with host are still unknown.In order to investigate the pathogen of C. cassiicola,the RFP-labeled transformant was generated with Agrobacterium tumefaciens-mediated transformation(ATMT).The transformant was able to emit strong red fluorescence on mycelia and conidia during 6 passages of transformants on PDA.The biological determination and pathogenicity test demonstrated that there was no significant differences between the wild strain and the transformant.关键词
黄瓜棒孢叶斑病/多主棒孢/农杆菌介导法/RFPKey words
Cucumber Corynespora leaf spot/Corynespora cassiicola/Agrobacterium mediated/RFP引用本文复制引用
谢学文,张自心,武军,石延霞,柴阿丽,李宝聚..黄瓜棒孢叶斑病病原菌RFP标记转化株的构建[J].中国蔬菜,2018,(3):45-49,5.基金项目
国家自然科学基金项目(31401888),国家大宗蔬菜产业技术体系项目(CARS-25) （31401888）
