中国生物防治学报2018，Vol.34Issue(1)：44-51,8.DOI:10.16409/j.cnki.2095-039x.2018.01.004
褐飞虱两个酚氧化酶原基因的特性及细菌诱导后的表达分析
Characteristics and Bacterial-induced Expression Analysis of Two Prophenoloxidase Genes in The Brown Planthopper, Nilaparvata lugens (Hemiptera Delphacidae)
摘要
Abstract
This study aims to explore the developmental expression pattern and biological function of prophenoloxidase in Nilaparvata lugens.The cDNA sequences encoding NlPPO1 (GenBank accession number:ALE32751) and NlPPO2 (GenBank accession number:ALE32752) were obtained from RNA-seq transcriptome sequencing database and rapid amplification of cDNA ends (RACE).The sequence analysis showed that the full-length cDNA sequence of NlPPO1 is 2316 bp,which contains an open reading frame (ORF) of 2073 bp encoding 690 amino acid-residues.And the full-length cDNA sequence of NlPPO2 is 2738 bp,which contains an ORF of 2100 bp encoding 699 amino acid-residues.The expression patterns and the changes of NlPPO mRNA induced by injecting bacteria were analyzed by real-time quantitative PCR.The developmental expression patterns showed that NlPPO1 and NlPPO2 were highly expressed in the gut,with a low mRNA level in the epidermis cell and fat body,respectively.The NlPPO1 mRNA expression increased with time and the NlPPO2 mRNA expression remained at a relatively high level in 5th instar larvae stage.The NlPPO2 mRNA expression decreased with time and the NlPPO1 mRNA expression remained at a relatively low level in adult stage.The NlPPO1 mRNA expression level significantly increased while the NlPPO2 mRNA expression level did not change significantly at 24 h after injection of Escherichia coli in N.lugens.The NlPPO1 and NlPPO2 mRNA expression levels significantly increased at 24 h after injection of Bacillus subtilis in N.lugens.The results showed that there are some differences between NlPPO1 and NlPPO2 between developmental stages and tissues in N.lugens.And the mRNA expression levels of NlPPO1 and NlPPO2 differ in the immune responses to bacteria,suggesting that the two NlPPO genes have different biological functions.The results provide a good foundation for further research on immune responses of NlPPO in the brown planthopper.关键词
褐飞虱/酚氧化酶原/特性/表达模式/诱导表达Key words
Nilaparvata lugens/prophenoloxidase/characteristics/expression pattern/inducible expression分类
生物科学引用本文复制引用
陈静,张道伟,钱正敏..褐飞虱两个酚氧化酶原基因的特性及细菌诱导后的表达分析[J].中国生物防治学报,2018,34(1):44-51,8.基金项目
国家自然科学基金(31560511) （31560511）
贵州省科学技术基金(2014-2014) （2014-2014）
贵州省教育厅青年基金(2014-320) （2014-320）
