中国生物防治学报2018，Vol.34Issue(1)：71-78,8.DOI:10.16409/j.cnki.2095-039x.2018.01.008
转cry1Ab+cry1C双价抗虫水稻对二化螟的抗性评价
Evaluation of Transgenic cry1Ab+cry1C Rice Lines for Its Resistance to Chilo suppressalis
摘要
Abstract
Control efficacies of four transgenic rice lines,cry1Ab,cry1C,cry1Ab+cry1C and cry1C+cry1Ab,against the main target pest,Chilo suppressalis,were evaluated through detached rice tissues bioassay,and Cry1Ab and Cry1C protein contents in these transgenic lines were measured by enzyme-linked immunosorbent assays.The results showed cumulative feeding areas of C.suppressalis feeding on transgenic rice lines for 48 h and 96 h were significantly lower than those on non-transgenic rice line,MH63.Four insect-resistant transgenic rice lines presented very high resistant level against C.suppressalis at early developmental stage (seedling,tillering and elongation stages),while control efficacy oftransgenic lines obviously decreased at later growth stages (booting and maturing stages).Double insect-resistant genes pyramided rice lines exhibited the highest resistant level among the four transgenic lines,second followed by cry1Ab and cry1C lines.Both Cry1Ab and Cry1C protein levels showed significantly different in respective transgenic rice lines at five different developmental stages.Additively,Cry1Ab protein expressing 1evels in transgenic lines were significantly higher than Cry1C protein.Cry1Ab levels were shown firstly increased and then decreased with the increase of growth stages in single-gene line,however,they presented the gradually decreasing trend with the increasing developmental stages both in leaf and stem tissues in double-gene lines.Cryl C levels showed the gradually rising trend with the increasing developmental stages in leaf tissues both in single-and double-gene lines.Moreover,Cry1Ab or Cry1C levels did not exhibit the significant decrease in double-gene lines,when compared with that in single-gene lines.Therefore,two-gene pyramided plants have robust prospects for commercial use,because they not only provide the efficient control against the pests,but also delay the insect resistance evolution.These results will provide the scientific data and theoretical basis for environmental safety assessment of transgenic Bt crops.关键词
二化螟/转基因抗虫水稻/抗虫效率/杀虫蛋白表达Key words
Chilo suppressalis/insect-resistant transgenic rice/control efficacy/insecticidal protein expression分类
农业科技引用本文复制引用
陈耕,何珊,韩兰芝,彭于发..转cry1Ab+cry1C双价抗虫水稻对二化螟的抗性评价[J].中国生物防治学报,2018,34(1):71-78,8.基金项目
国家转基因生物新品种培育重大专项(2016ZX08011-001) （2016ZX08011-001）
国家自然科学基金(31572336) （31572336）
