中国生物防治学报2018，Vol.34Issue(1)：124-132,9.DOI:10.16409/j.cnki.2095-039x.2018.01.015
基于转录组分析的哈茨木霉thga3基因功能研究
Functions of Thga3 Gene in Trichoderma harzianum Based on Transcriptome Analysis
摘要
Abstract
thga3 gene encoding a type Ⅲ G protein α subunit Thga3 in Trichoderma harzianum Th-33 may participated in the regulation of growth,spore production,mycoparasitism,hydrophobic and chitinase activity.In order to clarify its function,Illumina RNA-seq technology (RNA-seq) was used to analyze the differentially expressed genes (DEGs) between the wild-type strain Th-33 and thga3 knockout mutant △thga3,and the expression of genes related to the G protein signaling pathways,cell wall degradation enzymes,and the spore production were analysed.Comparing to wild Th-33,three DEGs of G protein receptor proteins,three chitinase genes,one sporulation regulatory gene brlA encoding a nucleic acid binding protein and one hydrophobin gene Tha_09745 were down-regulated,and one G protein signal transduction protein gene coding RGS1 was up-regulated in △thga3.It was hypothesized that thga3 may regulate sporulation by regulating the expression of brlA,regulate the mycelial hydrophobicity by regulating the expression of Tha_09745,and regulate the chitinase activity and affect the expression of other proteins in the G protein signaling network,which may affect the antagonism and mycoparasitism,as well as other biological characteristics.This study lays a foundation for further exploration on the regulation of G protein signaling pathway in Trichoderma biocontrol characteristics and its mechanism.关键词
哈茨木霉/转录组测序/差异表达基因/Thga3Key words
Trichoderma harzianum/transcriptome sequencing/differentially expressed genes/Thga3分类
农业科技引用本文复制引用
丁洁,蒋细良,梅杰,孙青,李梅..基于转录组分析的哈茨木霉thga3基因功能研究[J].中国生物防治学报,2018,34(1):124-132,9.基金项目
国家自然科学基金(31371983,31071728) （31371983,31071728）
