中国生物防治学报2018，Vol.34Issue(1)：156-162,7.DOI:10.16409/j.cnki.2095-039x.2018.01.019
枯草芽胞杆菌YB-05对小麦全蚀病菌胞内酶活的影响
Effect of Bacillus subtilis YB-05 Fermentation Broth on Gaeumannomyces gramini Enzyme Activities
摘要
Abstract
Wheat take-all pathogen is one of the main pathogenic funguses which exert influences on yield and quality of wheat,there have previous studies to prove that biocontrol strain B.subtilis YB-05 inhibits the growth of wheat take-all pathogen.This study intended to study the changes of the related enzymes in wheat take-all pathogen which were induced by B.subtilis YB-05,and explored the inhibition mechanism ofB.subtilis YB-05 fermentation broth against wheat take-all pathogen.Wheat take-all pathogen was treated with B.subtilis YB-05 fermentation broth which the concentration reached MIC50,the effects of B.subtilis YB-05 fermentation broth on mycelium structure and morphology of wheat take-all pathogen were observed by microscope;CAT,POD,PPO,SOD and PAL activity of wheat take-all pathogen were detected through enzyme activity assay,at the same time the enzymatic activities ofmitochondrial enzyme complexes Ⅱ and Ⅲ of wheat take-all pathogen in vitro/vivo were studied.The results showed that B.subtilis YB-05 fermentation broth resulted in aberrant growth of wheat take-all pathogen;The enzymatic activities of PAL,POD,CAT,PPO and SOD in wheat take-all pathogen treated with B.subtilis YB-05 fermentation broth for 8 day were 24.52％,72.67％,81.81％,80.36％ and 112.48％,lower than the control group,respectively;The enzymatic activities of mitochondrial enzyme complexes Ⅱ and Ⅲwere not changed significantly with the control in vitro.However,the enzymatic activities of mitochondrial enzyme complexes Ⅱ and Ⅲ were lower 43.95％ and 55.87％ than the control group in vivo,respectively.B.subtilis YB-05 fermentation broth affected the gene expression by combining the related genes of mitochondrial enzyme complexes Ⅱ and Ⅲ in wheat take-all pathogen,and then reduced the respiratory metabolism of wheat take-all pathogen by inhibiting the mitochondrial enzyme complexes Ⅱ and Ⅲ enzymatic activity,and finally caused the growth inhibition.关键词
枯草芽胞杆菌/发酵液/小麦全蚀病菌/防御酶活性/线粒体复合酶Ⅱ和Ⅲ酶活性Key words
Bacillus subtilis/fermentation broth/wheat take-all pathogen/activities of defensive enzyme/mitochondrial enzyme complexes Ⅱ and Ⅲ enzymatic activity分类
农业科技引用本文复制引用
孙润红,徐俊蕾,杨丽荣,张洁,夏明聪,武超,薛保国,吴坤..枯草芽胞杆菌YB-05对小麦全蚀病菌胞内酶活的影响[J].中国生物防治学报,2018,34(1):156-162,7.基金项目
河南省农业科学院科研发展专项资金(201513117) （201513117）
河南省博士后基金(2015107) （2015107）
小麦主要病虫害绿色防控产品的研发与产业化(141100111100) （141100111100）
河南省基础与前沿基金(162300410156) （162300410156）
