中国现代医学杂志2018，Vol.28Issue(8)：6-12,7.DOI:10.3969/j.issn.1005-8982.2018.08.002
MicroRNA-34a对小鼠气道平滑肌细胞增殖和ORMDL3表达的影响
MicroRNA-34a inhibits proliferation of airway smooth muscle cells and expression of ORMDL3
赵龙 1王宁 1石晓岚 1刘翠翠1
作者信息
- 1. 陕西省西安市儿童医院 呼吸哮喘科,陕西 西安 710003
- 折叠
摘要
Abstract
Objective To explore the regulating effect of microRNA-34a (miR-34a) on airway smooth muscle cell (ASMC) proliferation and orosomucoid1-like protein 3 (ORMDL3) level in bronchial asthma model of mice. Methods The mouse asthma model was induced by ovalbumin (OVA).ASMCs were cultrued in vitro. MicroRNAs which were implicated with ORMDL3 were screened. miR-34a was overexpressed by miR-34a-mimic. Hematoxylin-eosin (HE) staining and MTT method were used for the detection of ASMC proliferation. Quantitative real-time PCR and Western blot were used for examining the expressions of ORMDL3. Results The level of miR-34a was markedly inhibited in the airway tissues of the OVA-induced mouse model (P < 0.05). The abnormal porliferation of the ASMCs was attenuated by miR-34a-mimic (P < 0.05). The OD value at 490 nm and the relative cell activity of the ASMCs were significantly downregulated by miR-34a-mimic (P < 0.05). The expression of ORMDL3 in the airway tissues of the asthma mice was significantly inhibited by miR-34a-mimic(P<0.05).In vitro, miR-34a-mimic also downregulated the ORMDL3 level in the ASMCs (P < 0.05). Conclusions miR-34a plays a role in inhibiting the proliferation of ASMCs and regulating the expression of ORMDL3, and it can provide new ideas for understanding the pathogenesis of asthma.关键词
microRNA-34a/气道平滑肌细胞/血清类黏蛋白1样蛋白3Key words
microRNA-34a/airway smooth muscle cell/orosomucoid1-like protein 3分类
医药卫生引用本文复制引用
赵龙,王宁,石晓岚,刘翠翠..MicroRNA-34a对小鼠气道平滑肌细胞增殖和ORMDL3表达的影响[J].中国现代医学杂志,2018,28(8):6-12,7.
