中国现代医学杂志2018，Vol.28Issue(8)：23-29,7.DOI:10.3969/j.issn.1005-8982.2018.08.005
ZNF267在膀胱癌中的表达及其对细胞增殖和转移的影响
Expression of ZNF267 in bladder cancer and its effect on proliferation and migration of TCC cells
摘要
Abstract
Objective To investigate the expression of zinc finger 267 (ZNF267) in transitional cell carcinoma (TCC), and its effect on proliferation, apoptosis, cell cycle, migration and invasion of TCC cells. Methods qRT-PCR was performed to detect the expression of ZNF267 in tissues and cells of TCC. ZNF267 expression of TCC cells was silenced by siRNA. After silencing the expression of ZNF267 in TCC cells, the cell proliferation was detected by CCK-8, cell apoptosis and cell cycle were detected by flow cytometry. Furthermore, the effect of ZNF267 on cell migration and cell invasion was detected by Transwell assay. Results The expression of ZNF267 was up-regulated in the TCC tissues and cells compared with the adjacent tissues and immortalized human bladder urothelial cells. Compared with the ZNF267-normal TCC cells, the proliferation of the TCC cells transfected with ZNF267 siRNA was significantly inhibited, but the cell apoptosis ability was significantly promoted. Furthermore, the cell cycle of the TCC cells transfected with ZNF267 siRNA was arrest at G1 stage, the cell migration and invasion were decreased in the TCC cells transfected with ZNF267 siRNA. Conclusions ZNF267 is significantly upregulated in TCC tissues and cells, and could promote the proliferation and migration of TCC cells.关键词
锌指蛋白267/膀胱移行细胞癌/增殖/转移Key words
zinc finger 267/TCC/proliferation/migration分类
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曹正,邱明星..ZNF267在膀胱癌中的表达及其对细胞增殖和转移的影响[J].中国现代医学杂志,2018,28(8):23-29,7.
