中国畜牧兽医2018，Vol.45Issue(2)：310-319,10.DOI:10.16431/j.cnki.1671-7236.2018.02.004
一株鸭源H1N6亚型禽流感病毒的分离鉴定及HA和NA基因序列分析
Isolation, Identification and Genetic Analysis of HA and NA Genes of H1N6 Subtype Avian Influenza Virus
摘要
Abstract
In order to understand the epidemic situation of avian influenza virus (AIV) in Sino-Vietnamese border Guangxi,we carried out surveillance of avian influenza in the live poultry market in this region.A strain of H1N6 subtype AIV A/Duck/Guangxi/F01/2016 was isolated from live-bird market in Guangxi-Vietnam border areas.HA and NA genes were sequenced and the genetic analysis of isolate was made with other sequences available in GenBank.The results indicated that HA gene showed 96.9％ nucleotide homology with A/sparrow/Guangxi/GXs-1/2012 (H1N2),and NA gene showed 98.2％ nucleotide homology with A/Pavo cristatus/Jiangxi/JA1/2016(H5N6).The sequence analysis of HA gene showed that the virus was categorized as low pathogenic AIV,with the typical HA gene cleavage site of PSIQSR ↓ GLF.As the same with other N6 isolates,there were 11 amino acids missing.In addition,the receptor affinity of the isolate was also tested in this study.The result showed that the virus preferentially bind to the sialic acid α-2,3-Gal receptor.These data indicated that A/Duck/Guangxi/F01/2016 could be low pathogenic AIV and recombinant from different subtype of AIV.关键词
禽流感病毒(AIV)/H1N6亚型/序列分析Key words
avian influenza virus (AIV)/H1N6 subtype/sequence analysis分类
农业科技引用本文复制引用
熊文婕,谢芝勋,李孟,曹国敏,覃海,严小东..一株鸭源H1N6亚型禽流感病毒的分离鉴定及HA和NA基因序列分析[J].中国畜牧兽医,2018,45(2):310-319,10.基金项目
广西兽医生物技术重点实验开放基金(16-380-45-B-1) （16-380-45-B-1）
广西科技基地和人才专项项目(桂科AD16380009) （桂科AD16380009）
防城港科技基地和人才专项(防科AC17005006) （防科AC17005006）
