中国畜牧兽医2018，Vol.45Issue(2)：320-329,10.DOI:10.16431/j.cnki.1671-7236.2018.02.005
陆川猪黑皮质激素受体4基因克隆及序列分析
Cloning and Sequence Analysis of MC4R Gene in Luchuan Pig
摘要
Abstract
This study was aimed to clone melanocortin-4 receptor(MC4R) gene of Luchuan pig and analyze its genetic structure with bioinformatic software.The recombination plasmid pMD18-T-MC4R was constructed with RT-PCR and cloning methods,and detected by colony PCR and sequencing.After successfully constructed the recombination plasmid pMD18-T-MC4R,the further analysis of physical and chemical properties,structure of protein and modification and the subcellular localization were proceeded.The results showed that the cloned MC4R gene fragment included a 999 bp whole length CDS and encoded 332 amino acids.Compared with MC4R gene of Sus scrofa submission in NCBI,there were four base mutations of MC4R gene in Luchuan pig,two mutations at 175 and 906 bp were the same sense mutations,the other two mutations at 110 and 278 bp were the mis-sense mutations,causing glutamic acid converted to glycine and valine converted to alanine in 37 and 93 amino acid residues,respectively.Homology analysis indicated that MC4R was evolutionarily conserved in different species.Seven transmembrane helical regions,several N-glycosylation sites and distinct hydrophobic region were found,but no signal peptide and potential phosphorylation exited in MC4R protein,which might distribute mainly in the endoplasmic reticulum and vacuolar.The elements in the second structure of encoded protein were α-helix,extended strand,β-turn and random coil.The Luchuan pig MC4R gene was successfully cloned in this study,and provided a foundation for further studying on developing and utilizing the local species Luchuan pig and its breeding.关键词
陆川猪/MC4R基因/克隆Key words
Luchuan pig/MC4R gene/clone分类
生物科学引用本文复制引用
谢婉,蒋钦杨,杨秀荣,梁晶,谢炳坤,刘明君,何剑雄,申玉建,陈宝剑,关志惠,张名媛,郭亚芬,兰干球..陆川猪黑皮质激素受体4基因克隆及序列分析[J].中国畜牧兽医,2018,45(2):320-329,10.基金项目
国家自然科学基金(31360544) （31360544）
