中国畜牧兽医2018，Vol.45Issue(2)：349-357,9.DOI:10.16431/j.cnki.1671-7236.2018.02.008
透明质酸钠与软骨下钻孔对兔软骨损伤修复的对比试验
A Contrast Experiment Between Sodium Hyaluronate Injection and Subchondral Bone Drilling Treatment of Rabbit Cartilage Injury
摘要
Abstract
The rabbit models of osteochondral defects were established and two methods of treatment with external injection of sodium hyaluronate and subchondral bone drilling were adopted,the repair effect and early repair mechanism of these two methods were compared to provide experimental basis for clinical application.The models of articular cartilage injury in 36 Harbin White rabbits were set up and randomly allocated to three groups:Self-healing group (negative control),medication group (sodium hyaluronate injection) and drilling group (subchondral bone drilling).Rabbits from each group were sacrificed 1,4,6 and 8 weeks after operation,RT-PCR method was adopted to detect Col Ⅰ,Col Ⅱ,Col X,Agg,and Runx2 genes expression of repaired articular cartilage tissues;Postoperative 8 weeks,cartilage specimen were observed with HE staining,safranin green staining and toluidine blue staining to analyze the repair effect and repair mechanism of the three groups.The results showed that the cartilage damage of the two experimental groups were significantly repaired,staining showed that the chondrocytes in repaired tissues increased with typical cartilage lacuna like structure,the positive staining of medication group was better than drilling group.The expression of cartilage specificity genes Agg and Col Ⅱ mRNA in medication group was significatly higher than that in drilling group (P＜0.05).The expression of Col X and Runx2 of drilling group were more active than medication group (P＜0.05).The expression of major components of fibrous tissue,Col Ⅰ,self-healing group was the highest,then in drilling group,medication group was the lowest.The results showed that medication and drilling could repair the defects of the whole layer cartilage defects in various degrees.The main component of the restorations was fibrous tissue in self-healing group,more hyaline cartilage were produced by treated with hyaluronic acid and a thin layer of fibrous cartilage covering the surface of repair tissues in drilling group.In conclusion,in the short-term repair of bone defect,the effect of sodium hyaluronate was better than that of drilling.关键词
兔/软骨损伤/透明质酸钠/软骨下骨钻孔Key words
rabbit/cartilage injury/sodium hyaluronate/subchondral bone drilling分类
医药卫生引用本文复制引用
别连钰,蒋茹,马宁,刘涛,姜岩,肖建华..透明质酸钠与软骨下钻孔对兔软骨损伤修复的对比试验[J].中国畜牧兽医,2018,45(2):349-357,9.基金项目
“十二五”农村领域国家科技计划课题(2015BAD12B00) （2015BAD12B00）
