中国畜牧兽医2018，Vol.45Issue(2)：439-447,9.DOI:10.16431/j.cnki.1671-7236.2018.02.019
2006-2016年中国羊口疮病毒的遗传演化分析
Genetic and Phylogenetic Analysis of Orf Virus Isolated in China from 2006 to 2016
摘要
Abstract
Orf disease,an important zoonotic infectious disease,is caused by the Orf virus (ORFV),which was worldwide distribution and causing serious public health impacts.In order to know the origin,molecular epidemiology and systematic research information of ORFV of China,82 Chinese ORFV strains were retrieved and performed the genetic phylogenetic analysis,and the homologous alignment of nucleotide and amino acid sequences of ORFV011 (B2L),ORFV020 (VIR),ORFV059 (F1L) and ORFV127 (vIL-10) were analyzed using DNAStar and Mega 5.0 softwares,the phylogenetic tree was constructed.The results showed that the ORFV011 (B2L) and ORFV059 (F1L) sequences of 82 ORFV strains shared 95.9％ to 100％ and 94.5％ to 100％ nucleotide identities and 86.5％ to 100％ and 94.0％ to 100％ deduced amino acid identities,respectively.The phylogenetic analysis revealed that Chinese ORFV strains with different region clustered in different branch,even in the same provinces.These results indicated that ORFV strains in China did not form a unique cluster having possibility of mutation occurrence at any time and other worldwide ORFV strains showed variation circulating in China.This was the important obstacles for prevention and control of ORFV.These results provided the basic data for prevention of Orf disease and development of effective vaccine.关键词
中国/羊口疮病毒(ORFV)/遗传演化分析Key words
China/Orf virus (ORFV)/genetic and phylogenetic analysis分类
农业科技引用本文复制引用
葛士坤,张凯照,鲁荣,刘健新,宁章勇..2006-2016年中国羊口疮病毒的遗传演化分析[J].中国畜牧兽医,2018,45(2):439-447,9.基金项目
广东省科技计划项目(2016A020210079) （2016A020210079）
