中国畜牧兽医2018，Vol.45Issue(2)：456-462,7.DOI:10.16431/j.cnki.1671-7236.2018.02.021
2个与HSP70基因连锁的微卫星座位与牛运输应激性状的关联分析
Correlation Analysis Between 2 Microsatellite Loci Linked to the HSP70 Gene and Transport Stress Traits in Beef Cattle
摘要
Abstract
The correlation between 2 microsatellite loci linked to the HSP70 gene and transport stress traits in beef cattle was analyzed in this study.120 12-month old health Simmental crossbred beef cattle with 250 kg average body weight were used in transport stress test.Two microsatellite loci (BMS468 and BM1258) linked to HSP70 gene mapped to chromosome 23 were selected to study genetic polymorphisms in Simmental crossbred beef cattle population,the relation between microsatellite polymorphisms and transport stress traits were also analyzed by least square fitting general linear model.The results showed that:In BMS468 microsatellite locus,five alleles were detected (128,134,140,146 and 154 bp),the dominant allele was 128 and 134 bp,the effective allele number (Ne),polymorphism information content (PIC) and genetic heterozygosity (He) were 3.66,0.68 and 0.73,respectively.The correlation analysis showed that BMS468 microsatellite locus had a significant effect on the 7 d (after transport) average daily gain and disease incidence,the 7 d (after transport) average daily gain of 128/128 bp genotype was the highest (P＜0.05),and the disease incidence of 134/128 bp genotype was the lowest (P＜0.05).In BM1258 microsatellite locus,six alleles were detected (99,101,103,113,117 and 119 bp),the dominant allele was 101 bp,the Ne,PIC and He were 4.30,0.73 and 0.77,respectively.The correlation analysis showed that BM1258 microsatellite locus had a significant effect on the disease incidence (P＜0.05),and the disease incidence of 99/99 bp genotype was the lowest (P＜0.05).In conclusion,the 2 microsatellite loci with a rich genetic polymorphisms were valuable potential genetic markers for marker-assisted selection of anti-transport stress traits in beef cattle,providing the scientific basis for the markers selection for the anti-transport stress characteristics of cattle.关键词
肉牛/运输应激/微卫星座位/遗传标记Key words
beef cattle/transport stress/microsatellite loci/genetic marker分类
农业科技引用本文复制引用
刘延鑫,孙宇,李业亮,翟磊,郝万清,高腾云..2个与HSP70基因连锁的微卫星座位与牛运输应激性状的关联分析[J].中国畜牧兽医,2018,45(2):456-462,7.基金项目
现代农业产业技术体系建设专项资金项目(CARS-37) （CARS-37）
中国博士后面上基金项目(2012M521396) （2012M521396）
河南省基础与前沿技术研究(162300410149) （162300410149）
