中国畜牧兽医2018，Vol.45Issue(2)：486-492,7.DOI:10.16431/j.cnki.1671-7236.2018.02.025
成都地区宠物犬感染犬瘟热病毒和犬呼吸道冠状病毒的分子流行病学调查
Molecular Epidemiologic Investigation on Infections of Canine Distemper Virus and Canine Respiratory Coronavirus in Pet Dogs in Chengdu Area
摘要
Abstract
In order to investigate the prevalence and distribution of canine distemper virus (CDV) and canine respiratory coronavirus (CRCoV) infections in pet dogs in Chengdu area,420 samples of nasal cotton swabs were collected from dogs with respiratory signs in eight animal hospitals in Chengdu area,and the molecular detection were carried out using the RT-PCR assay.From 420 samples,213 samples were detected as CDV positive with the positive rate of 50.71％,and 247 samples were detected as CRCoV positive with the positive rate of 58.81％;The positive rate of mixed detection of CDV and CRCoV was 41.19％.The results indicated that the infection of CDV and CRCoV in pet dogs in Chengdu area was serious,and the mixed infection rate was also high.The detection rate of CDV and CRCoV in pet dogs was different from age,sex,variety,season and immunity.The detection rates of CDV and CRCoV in 1 to 3 month-old puppies were the highest,up to 74.40％ and 79.20％,respectively;And in purebred dogs were 59.37％ and 62.22％,respectively,which were higher than other varieties;The detection rate of CDV was higher up to 56.19％ in spring,however the detection rate of CRCoV was higher up to 67.59％ in winter.The detection rates of CDV and CRCoV in non-vaccinated dogs were higher than other dogs which were 64.42％ and 63.46％,respectively.This study enriched the epidemiological data of CDV and CRCoV in pet dogs,and provided basic data for diagnosis and control of CDV and CRCoV infections in pet dogs in Chengdu area.关键词
犬传染性呼吸道疾病(CIRD)/犬瘟热病毒(CDV)/犬呼吸道冠状病毒(CRCoV)/分子流行病学Key words
canine infectious respiratory disease (CIRD)/canine distemper virus (CDV)/canine respiratory coronavirus (CRCoV)/molecular epidemiology分类
农业科技引用本文复制引用
张昕,黄坚,张萍,杨晓农..成都地区宠物犬感染犬瘟热病毒和犬呼吸道冠状病毒的分子流行病学调查[J].中国畜牧兽医,2018,45(2):486-492,7.基金项目
四川省科技培训项目(2016KZ0007) （2016KZ0007）
