中国畜牧兽医2018，Vol.45Issue(2)：493-500,8.DOI:10.16431/j.cnki.1671-7236.2018.02.026
不同方法检测鸡白痢沙门氏菌抗体消长规律的比较研究
A Comparative Study of Various Methods for the Detection of Dynamic Rule of the Antibody Against Salmonella Pullorum
摘要
Abstract
To compare and evaluate the different methods for detection Salmonella Pullorum (S.Pullorum) antibody,and to provide scientific guidance for the purification of breeding chicken farms,SPF chickens were inoculated with active and inactivated S.Pullorum.Serum samples were collected and detected by three different methods (rapid serum agglutination,micro-agglutination and ELISA),and Kappa test was applied to determine the concordance among different methods.The results showed that positive samples were detected earlier when using rapid serum agglutination than ELISA,but the later method had a longer detection period above the threshold on positive samples,which was more consistent with the dynamic rule of antibody in S.Pullorum positive chickens.It showed weak agreement among three different methods (Kappa value were 0.002 to 0.295).In conclusion,the study demonstrated that there were big differences among three methods for S.Pullorum antibody detection.ELISA might be the best choice in single S.Pullorum infection,because of its no false positive interference,longer detection period above the threshold on S.Pullorum positive samples and more consistent with the dynamic rule of antibody.关键词
鸡白痢沙门氏菌/人工感染/抗体/平板凝集试验/微量凝集试验/ELISA/Kappa检验Key words
Salmonella Pullorum/artificial infection/antibody/rapid serum agglutination test/micro-agglutination test/ELISA/Kappa test分类
农业科技引用本文复制引用
刘洋,李丹,王传彬,霍斯琪,刘玉良,顾小雪..不同方法检测鸡白痢沙门氏菌抗体消长规律的比较研究[J].中国畜牧兽医,2018,45(2):493-500,8.基金项目
种禽场疾病综合防控与净化技术体系评估、论证和推广研究(2016YFD0501609) （2016YFD0501609）
