中国畜牧兽医2018，Vol.45Issue(2)：528-535,8.DOI:10.16431/j.cnki.1671-7236.2018.02.030
林蛙养殖场地土壤菌群结构及潜在病原菌分析
Analysis of Soil Microbial Communities and Potential Pathogens in Rana dybowskii Farm
摘要
Abstract
The aim of this study was to understand the composition and structural characteristics of soil microbial of Rana dybowskii,changes in microbes after spraying probiotics and the number of potential pathogens.Three experimental groups were set up:Breeding farm group,probiotics spraying group and semi-artificial breeding group.And high-throughput sequencing was used for detection.A total of 191 588 valid sequences and 1 369 OTUs were obtained from three experimental groups,which belonged to 438 genera and 29 phyla by taxonomy.The number of bacteria in three groups was relatively large,and the diversity level was relatively high.The dominant bacteria on phylum level were Proteobacteria,Bacteroidetes,Cyanobacteria,Gemmatimonadetes,Chloroflexi,Actinobacteria,Acidobacteria and Firmicutes;The dominant bacteria on genus level were Flavobacterium,Thermomonas,Sphingopyxis,Porph yrobacter and norank_c__Cyanobacteria.The diversity of microbial community among the groups did not reach the significant level.The similarity of the microbial communities was high.The potential red-leg syndrome pathogenic bacteria in the soil were Aeromonas,Citrobacter,Chryseobacterium and Pseudomonas.The changes of soil microbes after spraying probiotics were not obvious.This study provided the basis theory for comprehensively understanding the composition and variation of soil microbes of the Rana dybowskii farm,exploring the types of beneficial microorganisms and the prevention and treatment of Rana dybowskii.关键词
东北林蛙/土壤/高通量/微生物Key words
Rana dybowskii/soil/high-throughput sequencing/microbial分类
农业科技引用本文复制引用
别佳,佟庆,胡宗福,崔立勇,刘笑凝,王洪斌..林蛙养殖场地土壤菌群结构及潜在病原菌分析[J].中国畜牧兽医,2018,45(2):528-535,8.基金项目
林蛙集约化养殖防疫技术研究(sgzjY2010025) （sgzjY2010025）
