中国畜牧兽医2018，Vol.45Issue(2)：536-543,8.DOI:10.16431/j.cnki.1671-7236.2018.02.031
微生物发酵床猪舍空气微生物种群结构的研究
Piggery Air Microbial Community of Microbial Fermentation Bed
摘要
Abstract
To explore the characteristics of air microorganism of piggery with microbial fermentation bed,and provide theoretical basis for the assessment of air quality of microbial fermentation bed,air samples were collected by the method of natural sedimentation.Air microorganism were cultured and identified based on 16S rRNA sequence analysis for investigating the diversity of piggery air microorganism.More than 60 strains were isolated and sequenced.Ultimately,27 species were selected for subsequent analysis.The results showed that,among these 27 species,there were 14 Bacillus (51.9％),5 Pseudomonas (18.5％),4 Staphylococcus (14.8％),1 Ochrobactrum,1 Paenibacillus,1 Lysinibacillus and 1 Luteimonas.5 species of opportunistic pathogen and 6 species of organic degradation bacteria were found among these airborne bacteria.The airborne microbes were significantly clustered into 6 categories by SPSS analysis:Bacteria only distrusted in downwind piggery,bacteria only distrusted in windward piggery,bacteria only distrusted in outside piggery,bacteria distrusted in both windward and downwind piggery,bacteria distrusted in both downwind and outside piggery,and bacteria distrusted in all these three places.The species of airborne bacteria in downwind piggery were more than those of outside piggery and windward piggery.There were 20 species in downwind piggery,including 15 specific species.The adjacent locations had similar microbial composition because the microorganisms transported in the form of aerosol.Microbial fermentation bed has rich diversity of air microbial taxa,and the distributions of air microbial are affected by the wind.关键词
微生物发酵床/空气微生物/自然沉降法/微生物多样性Key words
microbial fermentation bed/air microorganism/natural sedimentation method/microbial diversity分类
农业科技引用本文复制引用
陈倩倩,刘波,王阶平,车建美,张海峰,朱育菁..微生物发酵床猪舍空气微生物种群结构的研究[J].中国畜牧兽医,2018,45(2):536-543,8.基金项目
福建省重大专项项目农业科技重大专项(2015NZ0003-1) （2015NZ0003-1）
福建省公益类科研院所专项(2015R1018-8) （2015R1018-8）
福建省公益类科研院所专项(2015R1018-1) （2015R1018-1）
国家科技支撑计划(2012BAD14B15) （2012BAD14B15）
公益性行业(农业)科研专项(201303094) （农业）
