中国畜牧杂志2018，Vol.54Issue(2)：44-47,4.DOI:10.19556/j.0258-7033.2018-02-044
甲状腺素对猪小肠上皮细胞miR-let-7a基因表达及细胞增殖的影响
Effects of Thyroxine on miR-let-7a Gene Expression and Cell Proliferation in Small Intestinal Epithelial Cells
摘要
Abstract
miR-let-7a plays an important role in the differentiation,proliferation and apoptosis of animal cells.The impact of thyroid hormone is very extensive,almost every cell of the body is the target of thyroid hormone,which can promote the differentiation,growth and maturation of the tissue.In this study,The effects of thyroxine were investigatied (T4) on the regulation ofmiR-let-7a gene expression and proliferation of small intestinal epithelial cells.The cells were cultured in different concentrations of T4 (0,0.02,0.03,0.05,0.075,0.1,0.2 mmol/L).The results showed that the expression of let-7a increased with the increased dosage of T4 from 0 to 0.03 μmol/L,the expression decreased,from 0.03 to 0.2 μmol/L.The expression is significantly higher than that of other groups (P＜0.01) at the concentration is 0.03 mmol/L,while the cell proliferation rate was significantly lower than that of other concentration group (P＜0.05).The expression of let-7a was negatively correlated with the cell proliferation.关键词
小肠上皮细胞/甲状腺素/miR-let-7a/基因表达量/细胞增殖率Key words
Small intestine epithelial cells/Thyroid hormone/Mir-let-7a/Gene expression/Cell proliferation rate分类
农业科技引用本文复制引用
高慧珍,陈理星,黄敏婕,徐宁迎..甲状腺素对猪小肠上皮细胞miR-let-7a基因表达及细胞增殖的影响[J].中国畜牧杂志,2018,54(2):44-47,4.基金项目
国家科技支撑计划(2015BAD03B01) （2015BAD03B01）
国家自然科学基金(31272424) （31272424）
