中国畜牧杂志2018，Vol.54Issue(2)：60-65,6.DOI:10.19556/j.0258-7033.2018-02-060
维生素E对绵羊原代睾丸间质细胞睾酮合成的影响
Effect of Vitamin E on Testosterone Synthesis in Sheep Primary Leydig Cells
摘要
Abstract
The trial was conducted to determine the effect of vitamin E on testosterone synthesis in sheep primary leydig cells.Dorper×thin tailed Han male sheeps were slaughtered to obtain testicular tissue.Seperating the Leydig cells,and the cells were randomly divided into four groups,each with age of 2 months groups was treated with different concentrations of vitamin E (0,49,80,160 μg/mL).Enzyme (3β-HSD,17β-HSD,P450scc,CYP17) content and genes (3β-HSD,17β-HSD,P450scc,CYP17,StAR) relative expression related to testosterone synthesis were measured after the completion of the culture.The results showed that compared with control group,supplementionwith different doses of vitamin E tended to increase testosterone synthesis (P=0.061).The relative expression of P450scc mRNA and 3β-HSD mRNA (P＜0.05) and the enzyme content of P450scc and 3β-HSD (P＜0.05) were significantly increased by adding 40 μg/mL vitamin E.关键词
维生素E/睾酮合成/睾丸间质细胞/绵羊Key words
Vitamin E/Testosterone synthesis/Leydig cell/Ovine分类
农业科技引用本文复制引用
修朋,曲扬华,高月锋,刁志成,罗海玲..维生素E对绵羊原代睾丸间质细胞睾酮合成的影响[J].中国畜牧杂志,2018,54(2):60-65,6.基金项目
国家自然科学基金(31472119) （31472119）
