中国医科大学学报2017，Vol.46Issue(12)：1062-1066,5.DOI:10.12007/j.issn.0258-4646.2017.12.002
榄香烯对神经母细胞瘤增殖的抑制作用
Mechanism of Inhibition of Neuroblastoma Cell Proliferation by Elemene
摘要
Abstract
Objective To investigate the effect of elemene and the mechanism of this effect on the proliferation of neuroblastoma.Methods We treated cultured SK-N-SH cells with elemene and used the MTT assay to determine the inhibition of cell proliferation.We used RT-qPCR and Western blotting to measure the effect of elemene on mRNA and protein expression,respectively,of repressor element-1 silencing transcription factor (REST) in SK-N-SH cells and evaluated the mRNA expression of CCND1 and CCNE1 in SK-N-SH cells by using RT-qPCR.Results Elemene significantly inhibited the proliferation of SK-N-SH cells in a dose-and time-dependent manner (P =0.001 16).Moreover,the mRNA (P =0.000 38) and protein (P =0.003 39) expression of REST were markedly suppressed by elemene.Furthermore,elemene significantly reduced the mRNA expression of CCND1 (P =0.001 91) and CCNE1 (P =0.000 15),which are related to the cell cycle.Conclusion Elemene significantly suppressed the proliferation of neuroblastoma cells through the reduction of REST,CCND1,and CCNE1.关键词
榄香烯/神经母细胞瘤/神经元限制性沉默因子/CCND1/CCNE1Key words
elemene/neuroblastoma/repressor element-1 silencing transcription factor/CCND1/CCNE1分类
医药卫生引用本文复制引用
周景儒,赵婉妮,梁剑峰..榄香烯对神经母细胞瘤增殖的抑制作用[J].中国医科大学学报,2017,46(12):1062-1066,5.基金项目
国家自然科学基金(81301092) （81301092）
中国博士后科学基金(2013M540885) （2013M540885）
