中国医科大学学报2017，Vol.46Issue(12)：1082-1086,5.DOI:10.12007/j.issn.0258-4646.2017.12.006
乙肝病毒X蛋白结合蛋白对腺样囊性癌细胞株ACC-2生物学行为的影响
Effect of HBXIP Expression on the Biological Behavior of the Adenoid Cystic Carcinoma Cell Line ACC-2
摘要
Abstract
Objective To study the effect of hepatitis B vin1s X-interacting protein (HBXIP) on the proliferation,migration,and invasion of ACC-2 cells,and the possible mechanism of the PI3K/Akt signaling pathway involved.Methods The chemically synthesized HBXIP-siRNA plasmid was transfected into the ACC-2 cells.RT-PCR and Western blotting were performed to detect the expression of HBXIP in the ACC-2 cells.Cell proliferation was measured via MTT assay.The invasive and migratory abilities of the ACC-2 cells were evaluated via the transwell chamber assay and scratch test,respectively.Western blotting also detected the impact of HBXIP-siRNA on Akt,p-Akt,PI3K,p-PI3K,and S100A4 protein expression.Results HBXIP was highly expressed and HBXIP-siRNA was successfully transfected in ACC-2 cells.MTT results showed that the number of surviving cells in the experimental group was significantly lower than that in the control group (P＜0.05).The scratch test results showed that the mobility of the experimental group was significantly lower than that of the control group (P＜0.01).The transwell assay showed that the rate of cell invasion of the experimental group was significantly lower than that of the control group (P＜0.01).Finally,Western blotting results revealed that the expression of p-Akt,p-PI3K,and S 100A4 was relatively decreased in the experimental group when compared to that in the control group.Conclusion Silencing the HBXIP gene inhibited ACC-2 proliferation,invasion,and migration.关键词
乙肝病毒X蛋白结合蛋白/腺样囊性癌细胞株ACC-2/唾液腺/生物学功能/PI3K/Akt信号通路Key words
hepatitis B X-interacting protein/ACC-2 cell line/salivary gland/biological functions/PI3K/Akt signaling pathway分类
医药卫生引用本文复制引用
孟雪,齐晓宇,刘维贤,王瑶,王秋旭..乙肝病毒X蛋白结合蛋白对腺样囊性癌细胞株ACC-2生物学行为的影响[J].中国医科大学学报,2017,46(12):1082-1086,5.基金项目
辽宁省科学技术计划(2011010215-404) （2011010215-404）
