中国油料作物学报2018，Vol.40Issue(1)：1-9,9.DOI:10.7505/j.issn.1007-9084.2018.01.001
甘蓝型油菜转录因子FUS3的克隆、表达分析及其与脂肪酸组分的关系
Transcription factor FUS3 and its relation to fatty acid in Brassica napus
摘要
Abstract
To investigate the function of rapeseed transcription factor FUS3 on fatty acids,CDS sequences of BnaA2.FUS3 and BnaA6.FUS3(two copies of BnaFUS3 gene)were cloned from Brassica nupus'xiangyou15'. Their CDS were 927 bp and 948 bp,encoded 309 and 315 amino acids. Amino acid sequence variation occurred in the C-terminal. Hydrophilic proteins BnaA2.FUS3 and BnaA6.FUS3 belong to B3 family,are membrane protein with no transmembrane region. Homology showed that both BnaA2.FUS3 and BnaA6.FUS3 were homologous genes of BraFUS3 and AtFUS3. Fluorescent relative quantitative analysis showed that BnaA2. FUS3 was expressed in roots,stems,leaves and horns,and was relatively stable in seeds. The expression peak appeared in pod skin at 25d after pollination. BnaA6.FUS3 was seed-specific and the expression peak appeared at 35d after pollination. Both expressions of BnaA2.FUS3 and BnaA6.FUS3 showed similar M-type in pod skin. The relationship between BnaFUS3 expression and accumulation of fatty acids in seed showed that,both expression BnaA2. FUS3 and BnaA6.FUS3 were rapidly increased at 30 d after pollination,when each fatty acid increased into a rapid period. They changed little at 45 to 50 d after pollination,and the fatty acid accumulation was also slowed down and be-came stable. This indicated that BnaFUS3 had important influence on embryogenesis and development,and played an important role in fatty acid synthesis and lipid accumulation.关键词
甘蓝型油菜/BnaFUS3/基因克隆/表达/脂肪酸组分Key words
Brassica napus/BnaFUS3/gene cloning/expression/fatty acids分类
农业科技引用本文复制引用
韦云婷,彭烨,吴宁柔,冯韬,官春云..甘蓝型油菜转录因子FUS3的克隆、表达分析及其与脂肪酸组分的关系[J].中国油料作物学报,2018,40(1):1-9,9.基金项目
国家973计划项目(2015CB150206) （2015CB150206）
国家重点研发计划项目课题(2016YFD0101303) （2016YFD0101303）
