外周血miRNA应用于肿瘤早期诊断的研究进展
Advances in research of peripheral blood miRNAs in early diagnosis of tumors
摘要
Abstract
MicroRNA (miRNA) is non-coding RNA molecule consisting of 20-25 nucleotides.It plays an important role in regulation of tumorigenesis and progression,including proliferation,differentiation and apoptosis of cancer cells,which directly affect the progress of tumors.Peripheral blood miRNA is relatively more stable,and easier to acquired and detected than tissue miRNA.It is a new generation biomarker for early detection and early diagnosis of tumors.It is also one of the main development directions of research and application in precision medicine.Methods commonly used in peripheral blood miRNA detection are RT-PCR,electrochemical detection,NanoString Technologies,genechip and high-throughput sequencing etc.Multiple miRNAs in peripheral blood are the early diagnostic markers for non-small cell lung cancer,esophageal squamous cell carcinoma,pancreatic cancer,squamous cell carcinoma of the head and neck,ovarian cancer,colorectal cancer,breast cancer,prostate cancer and hematological malignancies.Combined detection of multiple peripheral blood miRNAs,as well as combined detection of tumor-specific miRNAand serological,imaging and other auxiliary methods,can improve the sensitivity and specificity of tumor diagnosis at early stage.关键词
肿瘤/微小RNA/标志物/早期诊断Key words
tumor/microRNA/marker/early diagnosis分类
医药卫生引用本文复制引用
刘洪璐,王熙才..外周血miRNA应用于肿瘤早期诊断的研究进展[J].中国肿瘤生物治疗杂志,2018,25(2):109-117,9.基金项目
国家自然科学基金资助项目(No.81560380,81460358,81060177) （No.81560380,81460358,81060177）
云南省科技厅-昆明医科大学应用基础研究联合专项资助项目(No.2014FB066) （No.2014FB066）
云南省卫生科技项目(No.2014NS023,2014NS024) （No.2014NS023,2014NS024）
云南省卫生计生委医学学科带头人培养计划项目(No.D-201601) （No.D-201601）
詹启敏院士工作站基金(No.2015IC002). Project supported by National Natural Science Foundation of China (No.81560380,81460358,81060177),Joint Special Fund from Yunnan Provincial Science and Technology Department-Kunming Medical University for Applied and Basic Research (No.2014FB066),Project of Medical and Health Technology Development Program of Yunnan Province (No.2014NS023,2014NS024),Academic Leaders Training Program of Yunnan Provincial Health and Family Planning Commission (No.D-201601),and the ZHAN Qimin Academician Workstation Project Fund (No.2015IC002) （No.2015IC002）
