中国肿瘤生物治疗杂志2018，Vol.25Issue(2)：118-124,7.DOI:10.3872/j.issn.1007-385x.2018.02.002
miR-92b通过调控EZH2基因的表达抑制食管癌细胞Eca109的增殖和侵袭
miR-92b suppresses growth and invasion of esophageal cancer Eca109 cells by regulating EZH2 gene expression
摘要
Abstract
Objective:To investigate the regulating effects of miR-92b on the expression of EZH2 (enhancer of zeste homolog 2) gene and the proliferation and invasion abilities of esophageal cancer (EC) cells.Methods:Fifteen cases of esophageal cancer tissues that preserved in the research center of the Fourth Hospital Affiliated to Heibei Medical University from January 2016 to January 2017 were selected for this study.The bioinformatics tool was used to predict the possible miRNAs that might target EZH2.The mimics of predicted miRNAs were transfected into human esophageal carcinoma cell lines Eca109,respectively.Then the regulation effect of miRNAs on EZH2 gene expression was validated by real-time PCR,Western blotting and dual luciferase reporter experiment.In the meanwhile,EZH2 over-expression plasmids were co-transfected into esophageal carcinoma Eca109 cells,and the effects of miRNAs and EZH2 expression changes on the proliferation,apoptosis,invasion and migration of esophageal carcinoma cells were detected by CCK-8 method,Flow Cytometry,Transwell Invasion and migration assay,respectively.Results:Bioinformatics analysis showed that miR-92b,let7a and miR-25 could combine with potential binding sites at 3'-terminal non-translation region of EZH2 gene.Real-time PCR results showed that only miR-92b was able to regulate the expression of EZH2,and miR-92b was negatively correlated to EZH2 in esophageal cancer (P＜0.01).Compared with mimic-NC,the expression of EZH2 mRNA,protein and luciferase activity in Eca109 cells after miR-92b mimic transfection was significantly down-regulated (both P＜0.01).However,miR-92b mimic transfection had no effect on the apoptosis of Eca 109 cells.Moreover,the proliferation,invasion and migration of Eca 109 cells were significantly inhibited after transfection with miR-92b-mimic (P＜0.01).In addition,after co-transfection with EZH2 over-expression plasmids,the effects of miR-92b-mimic on the proliferation,invasion and migration of Eca109 cells were significantly weakened (P＜0.01).Conclusion:miR-92b can inhibit the proliferation,invasion and migration of esophageal carcinoma cells,and its mechanism may be related to its target regulation ofEZH2.关键词
食管癌/miR-92b/zeste同源物增强子2基因/增殖/侵袭/迁移Key words
esophageal carcinoma(EC)/miR-92b/enhancer of zeste homolog 2 (EZH2) gene/proliferation/invasion/migration分类
医药卫生引用本文复制引用
刘飞,孟令娇,刘世娜,谷丽娜,李娟,张建东,吴云艳,桑梅香..miR-92b通过调控EZH2基因的表达抑制食管癌细胞Eca109的增殖和侵袭[J].中国肿瘤生物治疗杂志,2018,25(2):118-124,7.基金项目
河北省杰出青年基金资助项目(No.2016206410).Project supported by the Science Fund for Outstanding Youth of Hebei Province (No.2016206410) （No.2016206410）
