中国肿瘤生物治疗杂志2018，Vol.25Issue(2)：132-136,5.DOI:10.3872/j.issn.1007-385x.2018.02.004
重组诱导性质粒Egr1-XPO4的构建及其与5-FU联合对肝癌细胞SK-Hep1的协同抑制作用
Construction of recombinant plasmids Egr1-XPO4 and its synergic inhibition with 5-FU against hepatocarcinoma SK-Hep1 cells
摘要
Abstract
Objective:To construct recombinant plasmid Egr1-XPO4 and evaluate its synergic inhibition with 5-FU against hepatocarcinoma SK-Hep1 cells.Methods:The XPO4 gene was inserted into vector carrying promoter Egr1 to construct a new recombinant vector,Egr1-XPO4,which was then transfected into human hepatocarcinoma cell line SK-Hep1 and sensitized with chemotherapeutic drug 5-FU.Western blotting was adopted to examine the protein expression of XPO4;CCK assay was used to detect SK-Hep1 cell proliferation after transfection,and Flow Cytometry with Annexin V-FITC/PI staining was used to detect the apoptosis of SK-Hep1 cells.SK-Hep1 cell xenograft model was constructed on nude mice,and the effect of Egr1-XPO4 in combination with 5-FU on the growth of xenograft was observed.Results:The recombinant plasmid Egr1-XPO4 was successfully constructed.With the sensitization of 5-FU,the expression of XPO4 protein in SK-Hep1 cells was significantly elevated after Egr1-XPO4 transfection,and the evlevation was in a 5-FU dose-depend manner.The combined treatment of Egr1-XPO4 and 5-FU produced a significantly stronger inhibition against SK-Hep1 cell proliferation and greatly promoted apoptosis of SK-Hep1 cells compared with 5-FU or pEgr-XPO4 mono-treatment group (all P＜0.05).And in vivo antitumor experiment showed that the tumor volume in Egr1-XPO4 + 5-FU treatment group was significantly smaller than that of Egr1-XPO4 or 5-FU mono-treatment group (P＜0.05).Conclusion:The recombinant plasmid Egr1-XPO4 in combination with 5-FU could exertsynergic inhibitionagainst hepatocarcinomaSK-Hep 1 cells.关键词
肝癌/SK-Hep1细胞/XPO4基因/Egr1启动子/5-氟尿嘧啶/协同作用Key words
hepatocarcinoma/SK-Hep1 cell/XPO4 gene/Egr1 promoter/5-FU/synergic effect分类
医药卫生引用本文复制引用
吴楠,黄静,陈阳,刘侃,刘好,王晓梅..重组诱导性质粒Egr1-XPO4的构建及其与5-FU联合对肝癌细胞SK-Hep1的协同抑制作用[J].中国肿瘤生物治疗杂志,2018,25(2):132-136,5.基金项目
深圳国际合作研究资助项目(No.GJHZ20170313111237888). Project supported by the Shenzhen International Cooperation Research Project(No.GJHZ20170313111237888) （No.GJHZ20170313111237888）
