中国肿瘤生物治疗杂志2018，Vol.25Issue(2)：142-147,6.DOI:10.3872/j.issn.1007-385x.2018.02.006
过表达IL-18抑制人结直肠癌细胞HCT-116的增殖
IL-18 over-expression inhibits proliferation of human colorectal cancer HCT-116 cells
摘要
Abstract
Objective:To investigate the effects of interleukin-18 over-expression on the in vitro and in vivo proliferation of human colorectal cancer (CRC) HCT-116 cells.Methods:A recombinant lentivirus vector containing human IL-18 gene fragment was constructed.Then theCRC HCT-116 cell line stably expressing human IL-18 (HCT-116/IL-18) was obtained by recombinant lentivirus transfection.In vitro proliferation of HCT-116/IL-18 cells and wild-type HCT-116 cells was determined by C CK-8 method.The expressions of IL-18,Cyclin D 1,proliferating cell nuclear antigen (PCNA) and DNA damage repair enzyme (PARP) were detected by Western blotting.HCT-116 and HCT-116/IL-18 cells were inoculated into left and right axillas of Balb/c nude mice,respectively.Then the tumorigenicity and the growth of transplanted tumor were observed.The expressions ofIL-18 and PCNA in xenograft tissues were detected by immunohistochemistry analysis.Results:IL-18 gene over-expression in HCT-116 cells could delay the proliferation of HCT-116 cells (P＜0.05 or P＜0.01).PARP expression was increased significantly and PCNA,Cyclin D1 expression were decreased in HCT-116/ IL-18 cells as compared to that of HCT-116 cells (P＜0.01).The tumorigenicity of HCT-116/IL-18 cell was significantly decreased in nude mice with a tumor-formation rate of 43％;Compared with control group,HCT-116/IL-18cell line had a longer tumorigensis time,slower growth and smaller tumor volume;moreover,PCNA protein expression was down-regulated in HCT-116/IL-18 xenograft tissuesas shown by immunohistochemistry analysis (P＜0.01).Conclusion:IL-18 over-expression inhibited the growth and proliferation of HCT-116cells both in vitro andin vivo,and the mechanism might be related with IL-18 regulating cell cycle and promoting DNA damage.关键词
结直肠癌/白介素-18/细胞增殖/移植瘤Key words
colorectal cancer(CRC)/interlukin-18 (IL-18)/proliferation/transplanted tumor分类
医药卫生引用本文复制引用
陈亚楠,杨如,许芝山,吴敏娜,路平,钟根深..过表达IL-18抑制人结直肠癌细胞HCT-116的增殖[J].中国肿瘤生物治疗杂志,2018,25(2):142-147,6.基金项目
国家自然科学青年基金资助项目(No.81201765) （No.81201765）
河南省高校重点科研资助项目(No.15A320063,No.16A310022) （No.15A320063,No.16A310022）
河南省骨干青年教师培养计划(No.2016GGJS-105) （No.2016GGJS-105）
新乡医学院研究生科研创新支持计划资助项目(No.YJSCX201616Y). Project supported by the National Natural Science Foundation of China (No.81201765),the Key Scientific Research Projects for Higher Education of Henan Province (No.15A320063,No.16A310022),the Young Backbone Teachers Training Program of Henan Province (No.2016GGJS-105),and the Graduate Student Support of Scientific Research Innovation Projects of Xinxiang Medical University (No.YJSCX201616Y) （No.YJSCX201616Y）
