中成药2018，Vol.40Issue(2)：341-346,6.DOI:10.3969/j.issn.1001-1528.2018.02.018
HPLC法同时测定加味二妙颗粒中7种成分及指纹图谱建立
Simultaneous determination of seven constituents in Supplemented Ermiao Granules by HPLC and establishment of fingerprints
摘要
Abstract
AIM To establish an HPLC method for the simultaneous content determination of phellodendrine chloride,berberine hydrochloride,jatrorrhizine hydrochloride,(R,S)-goitrine,astilbin,hesperidin and atractylenolide Ⅲ in Supplemented Ermiao Granules (Phellodendri chinensis Cortex,Atractylodis Rhizoma,Smilacis glabrae Rhizoma,etc.) and to establish fingerprints.METHODS The analysis of 70％ methanol extract of this drug was performed on a 35 ℃ thermostatic Amethyst C18-H column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of 0.05 mol/L potassium dihydrogen phosphate-acetonitrile flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 237,291 nm.RESULTS Seven constituents showed good linear relationships within their own ranges (r =1),whose average recoveries were 95.77％-110.36％ with the RS-Ds of 0.06％-2.94％.There were twenty common peaks in the fingerprints of fourteen batches of samples with the similarities of more than 0.990,five of which (5-hydroxymethylfurfural,phellodendrine,astilbin,hesperidin and berberine hydrochloride) were identified.CONCLUSION This stable,reliable and reproducible method can be used for the quality control of Supplemented Ermiao Granules.关键词
加味二妙颗粒/化学成分/指纹图谱/HPLCKey words
Supplemented Ermiao Granules/chemical constituents/fingerprints/HPLC分类
医药卫生引用本文复制引用
邹静,陈慧,彭懿,任青玲,刘志辉..HPLC法同时测定加味二妙颗粒中7种成分及指纹图谱建立[J].中成药,2018,40(2):341-346,6.基金项目
第二周期江苏省妇幼健康重点人才项目(FRC201711) （FRC201711）
