中成药2018，Vol.40Issue(2)：347-350,4.DOI:10.3969/j.issn.1001-1528.2018.02.019
HPLC法同时测定外搽白灵酊中7种成分
Simultaneous determination of seven constituents in Waicha Bailing Tincture by HPLC
陈红凯 1裴翰臻 2赵海龙3
作者信息
- 1. 青海大学附属医院药剂科,青海西宁810001
- 2. 青海制药厂有限公司,青海西宁810003
- 3. 青海大学医学院,青海西宁810001
- 折叠
摘要
Abstract
AIM To eslablish an HPLC method for the simultaneous content determination of brazilin,(±) protosappanin B,hydroxysafflor yellow A,safflor yellow A,oxypeucedanin,imperatorin and isoimperatorin in Waicha Bailing Tincture (Angelicae dahuricae Radix,Sappan Lignum,Carthami Flos,etc.).METHODS The analysis of methanol extract of this drug was performed on a Agilent TC-C18 column (200 mm ×4.6 mm,5 μm),with the mobile phase comprising of methanol-0.1％ glacial acetic acid flowing at 0.9 mL/min in a gradient elution manner,and the detection wavelengths were set at 285,403,310 nm.RESULTS Seven constituents showed good linear relationships within their own ranges (r ≥0.999 5),whose average recoveries were 96.91％-99.59％ with the RSDs of 0.81％-1.19％.CONCLUSION This stable and reliable method can be used for the quality control of Waicha Bailing Tincture.关键词
外搽白灵酊/化学成分/HPLCKey words
Waicha Bailing Tincture/chemical constituents/HPLC分类
医药卫生引用本文复制引用
陈红凯,裴翰臻,赵海龙..HPLC法同时测定外搽白灵酊中7种成分[J].中成药,2018,40(2):347-350,4.
