中成药2018，Vol.40Issue(2)：355-358,4.DOI:10.3969/j.issn.1001-1528.2018.02.021
HPLC法同时测定六经头痛片中4种成分
Simultaneous determination of four constituents in Liujing Toutong Tablets by HPLC
摘要
Abstract
AIM To establish an HPLC method for the simultaneous content determination of four constituents in Liujing Toutong Tablets (Angelicae dahuricae Radix,Magnoliae Flos,Ligustici Rhizoma et Radix,etc.).METHODS The analysis of 30％ ethanol extract of this drug was performed on a 35 ℃ thermostatic Waters C18 column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of methanol-4％ acetic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 320 nm.RESULTS Puerarin,ferulic acid,imperatorin and isoimperatorin showed good linear relationships within the ranges of 60.6-303 μg/mL (r=0.999 9),1.59-7.95 μg/mL (r =0.999 9),1.57-7.85 μg/mL (r =0.999 9) and 0.752 5-3.762 5 μg/mL (r =0.999 7),whose average recoveries (RSDs) were 97.75％ (1.7％),97.68％ (2.3％),97.94％ (1.0％) and 98.29％ (1.6％),respectively.CONCLUSION This stable and reliable method can be used for the quality control of Liujing Toutong Tablets.关键词
六经头痛片/葛根素/阿魏酸/欧前胡素/异欧前胡素/HPLCKey words
Liujing Toutong Tablets/puerarin/ferulic acid/imperatorin/isoimperatorin/HPLC分类
医药卫生引用本文复制引用
董虹玲,李钦青,柴金苗,贺文彬,李秀英..HPLC法同时测定六经头痛片中4种成分[J].中成药,2018,40(2):355-358,4.基金项目
山西省科技创新团队建设计划项目(2013131022) （2013131022）
国家自然科学基金资助项目(81681260247) （81681260247）
