中成药2018，Vol.40Issue(2)：363-368,6.DOI:10.3969/j.issn.1001-1528.2018.02.023
金丝草化学成分及其体外抗HBV活性
Chemical constituents from Pogonatherum crinitum and their in vitro anti-HBV activities
摘要
Abstract
AIM To study the chemical constituents from Pogonatherum crinitum (Thunb.) Kunthand and their in vitro anti-HBV activities.METHODS The ethyl acetate and n-butanol fractions of 70％ ethanol extract from P.crinitum were isolated and purified by AB-8 macroporous resin,silica column and Sephadex LH-20,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The in vitro anti-HBV activities of the isolated compounds were tested in the model of HepG2.2.15 cells.RESULTS Eight compounds were isolated and identified as 6,8,4'-trihydroxy-7,3'-dimethoxyisoflavone (1),quercetin-7,4'-dimethylester-5-O-β-D-glucopyranoside (2),8-(1-(3,4-dihydroxyphenyl) ethyl) quercetin (3),chrysoeiml-7-O-α-L-rhamnopyranosyl-(1 → 2)-β-D-glucopyranoside (4),kaempferol-3-O-(2",3"-di-O-E-p-cou-maroyl)-α-L-rhamnopyranoside (5),1,3,7-trihydroxyxanthone-2-C-β-D-glucopyra-noside (6),luteolin-6-C-β-D-boivinopyranoside (7),luteolin-6-C-β-D-glucopyranoside (8).Compounds 3,5,7,8 had certain inhibitory effects on HBsAg with the IC50 values of 96.6,127.1,72.4 and 54.5 μg/mL.Compounds 7,8 had certain inhibitory effects on HBeAg with the IC50 values of 81.0,61.3 μg/mL.CONCLUSION Compounds 1-6 are isolated from genus Pogonatherum for the first time,compounds 3、5、7、8 have certain in vitro anti-HBV activities.关键词
金丝草/化学成分/分离鉴定/体外抗HBV活性Key words
Pogonatherum crinitum (Thunb.) Kunth/chemical constituents/isolation and identification/in vitro anti-HBV activities分类
医药卫生引用本文复制引用
袁晓旭,李洪波,尹志峰,王慧娜,赵桂琴..金丝草化学成分及其体外抗HBV活性[J].中成药,2018,40(2):363-368,6.基金项目
河北省教育厅重点项目(ZD2016061) （ZD2016061）
河北省中医药管理局项目(2015034) （2015034）
河北省高校重点学科建设项目(冀教高[2013]4) （冀教高[2013]4）
