中成药2018，Vol.40Issue(2)：383-387,5.DOI:10.3969/j.issn.1001-1528.2018.02.027
紫地榆HPLC指纹图谱建立及模式识别
Establishment of HPLC fingerprints of Geranium strictipes and pattern recognition
摘要
Abstract
AIM To establish HPLC fingerprints of Geranium strictipes R.Kunthand to perform pattern recognition.METHODS The analysis of 70％ methanol extract of G.strictipes was performed on a 25℃ thermostatic ZORBAX SB-C18 column(250 mm× 4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-water in a gradient elution manner,and the detection wavelength was set at 254 nm.The results were analyzed by cluster analysis and principal component analysis.RESULTS There were thirteen common peaks in the HPLC fingerprints of twenty-one batches of samples with the similarities of more than 0.894.Twenty-one batches of samples were divided into two categories.The cumulative contribution rate of the first principal component was 31.067％.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of G.strictipes.关键词
紫地榆/HPLC指纹图谱/聚类分析/主成分分析Key words
Geranium strictipes R.Kunth/HPLC fingerprints/cluster analysis/principal component analysis分类
医药卫生引用本文复制引用
杨晓珍,谢苗,王燕,周萍,蓝海..紫地榆HPLC指纹图谱建立及模式识别[J].中成药,2018,40(2):383-387,5.基金项目
国家自然科学基金(81260512) （81260512）
云南省大理学院应用开发研究基金(Kyyy201102) （Kyyy201102）
