中药材2017，Vol.40Issue(9)：2156-2160,5.DOI:10.13863/j.issn1001-4454.2017.09.036
壮药毛果鱼藤提取物体内外降糖作用的初步研究
Study on Hypoglycemic Effect of Derris eriocarpa Extraction in vitro and in vivo
摘要
Abstract
Objective:To investigate the hypoglycemic effect of the extraction from Derris eriocarpa in vivo and in vitro.Methods:The glucose consumption(GC) and cell proliferation in high glucose model HepG2 cell culture medium were measured.Diabetic mice were induced by intravenous injection of Streptozotocin.Diabetic mice were randomly divided into model group,Metformin positive control group,and low-,middle-,high-dose (200,400,800 mg/kg)Derris eriocarpa groups,and normal control group were setup.Diabetic mice were intragastric (ig)corresponding drugs for 21 d.Fasting blood glucose (FBG)was measured weekly.After 21 d,pancreatic index,liver index,area under the curve (AUC),and the level of serum superoxide dismutase (SOD),malondialdehyde (MDA),glutathione peroxidase (GSH-Px) were measured.Pathological changes of pancreas were observed,immunohistochemical analysis was used to detected the expression of Caspase-3 and NF-κB in the pancreas tissues.Results:Hypoglycemic effect of petroleum ether extract in vitro was the optimum selection,the expression of FBG,MDA,AUC,Caspase-3,NF-κB of model mice were significantly decreased in vivo.The activity of SOD,GSH-Px in were increased(P＜0.05 or P＜0.01).The pathological changes of pancreas were improved.Conclusion:The petroleum ether extract of Derris eriocarpa has good hypoglycemic effect in vivo and in vitro.关键词
毛果鱼藤/HepG2细胞/2型糖尿病/降糖作用Key words
Derris eriocarpa How./HepG2 cells/Type 2 diabetes mellitus/Hypoglycemic effect分类
医药卫生引用本文复制引用
邓丽姚,姜明国,杨立芳,黄仁彬,石松标,郭洋洋,吴霜..壮药毛果鱼藤提取物体内外降糖作用的初步研究[J].中药材,2017,40(9):2156-2160,5.基金项目
国家自然科学基金(81560713) （81560713）
广西自然科学基金(2016GXNSFAA380075) （2016GXNSFAA380075）
