中药材2017，Vol.40Issue(9)：2161-2165,5.DOI:10.13863/j.issn1001-4454.2017.09.037
苦参碱抑制结肠癌细胞生长和迁移侵袭的作用研究
Suppressive Effect of Matrine on Cell Growth, Migration and Invasion of Colon Cancer Cells
摘要
Abstract
Objective:To investigate the effect of matrine on proliferation,colony formation,migration,invasion and AKT/GSK3β/ β-catenin signaling pathway of SW480 colon cancer cells.Methods:The cell proliferation was detected by MTT assay.The cell colony formation ability was assessed by colony formation assay.The ability of migration and invasion was evaluated by Transwell assay.The protein expressions of AKT,GSK3β,β-catenin and phosphorylation of protein were analyzed by Western blotting.Results:Matrine could inhibit the proliferation of SW480 cells and SW480/M5 cells in a concentration and time-dependent manner.The IC50 of SW480 cells were 1 919,832.8 and 318.1 μg/mL at 24,48 and 72 h,and the IC50 of SW480/M5 cells were 2 417,947 and 581 μg/mL.The colony formation of SW480 and SW480/M5 cells was decreased by matrine in a concentration-dependent manner,and matrine can inhibit the migration and invasion of SW480 cells and SW480/M5 cells.800 μg/mL matrine inhibited the phosphorylation of AKT and GSK3β protein of SW480 and SW480/M5 cells,and induced the phosphorylation degradation ofβ-catenin.Conclusion:Matrine can inhibit the proliferation,colony formation,migration and invasion of SW480 cells and SW480/M5 cells,and the mechanism may be related to the inhibition of AKT/GSK3β/β-catenin signaling pathway.关键词
苦参碱/结肠癌/增殖/迁移/侵袭/β-cateninKey words
Matrine/Colon cancer/Proliferation/Migration/Invasion/β-catenin分类
医药卫生引用本文复制引用
李金州,陈娜娜,戴美琴,郭丹..苦参碱抑制结肠癌细胞生长和迁移侵袭的作用研究[J].中药材,2017,40(9):2161-2165,5.基金项目
广东省自然科学基金(2016A030313595) （2016A030313595）
广州市科技计划项目(201707010074) （201707010074）
