中药材2017，Vol.40Issue(9)：2166-2171,6.DOI:10.13863/j.issn1001-4454.2017.09.038
葶苈子对肾阴虚水肿模型干预作用的代谢组学研究
Metabonomic Study on the Effect of Descurainia sophia Seeds for Kidney Yin Deficiency Edema in Rats
摘要
Abstract
Objective:To research the kidney yin deficiency edema and to assess the integral efficacy of Descurinia sophia seeds in rat model by metabonomics.Methods:UPLC-Q-TOF-MS method was used to detect the changes of urine metabolites in all groups.Principal component analysis(PCA) and Orthogonal partial least squares discriminant analysis(OPLS-DA) were utilized to analyze and screen potential biomarkers.MeV software was adopted for heat map drawing and cluster analysis.Results:40 biomarkers which identified were mainly related to four metabolic pathways that were significantly disrupted(Phenylalanine,Tryptophan,Histidine and Pyrimidine).The mostly biomarker levels in the seeds of Descurinia sophia group exhibited a reverse to normal status,metabolic disorders can be corrected.Conclusion:Metabolomics describes the pathological status of kidney yin deficiency edema and the effect of the seeds of Descurinia sophia from an overall level,which provides a powerful approach for illustrating the Chinese medicine syndrome and action mechanism of Chinese herbal medicine.关键词
代谢组学/葶苈子/肾阴虚水肿/热度图/代谢通路Key words
Metabonomics/The seeds of Descurainia sophia(L.)Webb.ex Prantl./Kidney yin deficiency edema/Heat map/Metabolic pathway分类
医药卫生引用本文复制引用
周宁,郑晓珂,王秋红,匡海学,冯卫生,牛艳..葶苈子对肾阴虚水肿模型干预作用的代谢组学研究[J].中药材,2017,40(9):2166-2171,6.基金项目
国家重点基础研究发展计划(973计划,2013CB531802) （973计划,2013CB531802）
