中药材2017，Vol.40Issue(9)：2177-2181,5.DOI:10.13863/j.issn1001-4454.2017.09.040
肾气丸对衰老大鼠小肠干细胞及其微环境Wnt/β-catenin信号通路的调节作用
The Effect of Shenqi Pills on Small Intestinal Stem Cells and Microenvironment Wnt/β-catenin Signaling Pathway in Aging Rats
摘要
Abstract
Objective:To explgre the effect of Shenqi pills on small intestinal stem cells and the microenvironment of Wnt/β-catenin signaling pathway in aging rats.Methods:SD rats were divided into six month age as adult control group,22 month age as aging model group,Shenqi pill low-dose group,Shenqi pill high-dose group.The morphology of small intestine was determined by HE staining and the corresponding indexes were measured.The expression of PCNA,Bmi 1,β-catenin and GSK-3β in small intestine was detected by immunohistochemistry.Results:Compared with the adult control group,the villus length,villus width,crypt depth,and villus/crypt value (V/C) in aging group were decreased significantly.The expression levels of PCNA,Bmi 1 and β-catenin were decreased significantly,while the expression of GSK-3β was increased significantly in the intestine of the aging group.Compared with aging group,the villus length,villus width,crypt depth and villus/crypt value(V/C) of shenqi pill groups were increased significantly.The expression levels of PCNA,Bmi 1 and β-catenin were increased significantly,while the expression of GSK-3β was decreased significantly in the intestine of the aging group.Conclusion:Shenqi Pills can improve the proliferation of intestinal stem cells and upregulate Wnt/β-catenin signaling pathway in aging rats.关键词
肾气丸/衰老/小肠干细胞/Wnt/β-catenin信号通路Key words
Shenqi pills/Aging/Intestinal stem cells/Wnt/β-catenin signaling pathway分类
医药卫生引用本文复制引用
彭丹丽,郭煜晖,李玉婷,袁丁,张长城,顿耀艳..肾气丸对衰老大鼠小肠干细胞及其微环境Wnt/β-catenin信号通路的调节作用[J].中药材,2017,40(9):2177-2181,5.基金项目
国家自然科学基金(81503423,81403307) （81503423,81403307）
