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正交试验设计优化木瓜多糖提取工艺

李宝珠 苏诗蟠 王中来 胡育梅

安徽农业科学2018,Vol.46Issue(9):134-136,3.
安徽农业科学2018,Vol.46Issue(9):134-136,3.

正交试验设计优化木瓜多糖提取工艺

Optimization of Polysaccharide Extraction from Papaya by Orthogonal Experiment Design

李宝珠 1苏诗蟠 2王中来 3胡育梅1

作者信息

  • 1. 福州大学生物科学与工程学院,福建福州350116
  • 2. 泉州医学高等专科学校检验预防学院,福建泉州362000
  • 3. 厦门伯赛基因转入技术有限公司,福建厦门361002
  • 折叠

摘要

Abstract

[Objective] To optimize the extraction process of papaya polysaccharide by ultrasonic assisted hot water extraction, and improve the yield. [Method]The effects of four factors such as ultrasonic treatment time A (min), extraction time B (min), solid-liquid ratio C (mg/mL) and extraction temperature D (℃),on the yield of polysaccharide were investigated by L27(313) orthogonal experiment, taking into account the possible interactions AB,AC and AD. Finally,the optimal level combination was found.[Result]By considering the interac-tion and designing the four-factor three-level orthogonal test using L27(313),the optimal combination of technological level is A3B2C1D3,that is,ultrasonic treatment time 40 min,extraction time 60 min,solid-liquid ratio 1:30 mg/mL,and the extraction temperature was 80 ℃. The optimal yield of papaya polysaccharide was 15.2% under the optimal combination condition.[Conclusion] The optimal process combination of polysaccharide extraction from papaya was determined,which provided technical guidance for the development and utilization of papaya poly-saccharide.

关键词

木瓜多糖/正交试验/交互作用/方差分析

Key words

Papaya polysaccharide/Orthogonal experiment/Interaction/Variance analysis

分类

农业科技

引用本文复制引用

李宝珠,苏诗蟠,王中来,胡育梅..正交试验设计优化木瓜多糖提取工艺[J].安徽农业科学,2018,46(9):134-136,3.

基金项目

福建省自然科学基金项目(2015J01135). (2015J01135)

安徽农业科学

0517-6611

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