吉林大学学报（医学版）2018，Vol.44Issue(2)：211-215,封2,6.DOI:10.13481/j.1671-587x.20180202
Lyn对屋尘螨诱导的人支气管上皮细胞中MUC5AC表达的影响及其机制
Effect of Lyn on expression of MUC5AC in human bronchial epithelial cells induced by house dust mite and its mechanism
摘要
Abstract
Objective:To investigate the effect of Lyn on the expression of MUC5AC in human bronchial epithelial cells induced by house dust mite(HDM),and to explore its possible mechanism.Methods:The human bronchial epithelial cells(16 HBE)were divided into PBS group and HDM group(1 μg·L-1HDM).The cells were transfected by liposome.The luciferase report gene of MUC5AC promoter was constructed.The relative luciferase unit(RLU)was detected by double luciferase report gene assay.The expression of MUC5AC in the cells was detected by immunofluorescence technique and observed by confocal microscope.The expression level of STAT6 in the 16HBE cells was detected by Western blotting method.Results:The results of double luciferase report gene assay showed that the RLU in HDM group was higher than that in PBS group(P<0.05).The RLU of cells in HDM group treated with LynsiRNA intervention was higher than that of the cells without LynsiRNA intervention(P<0.05).The immunofluorescence results demonstrated that the expression level of MUC5AC in HDM group was higher than that in PBS group(P<0.05),and the expression level of MUC5AC in HDM group was increased after LynsiRNA intervention(P<0.05).The Western blotting results indicated that the expression level of STAT6 was up-regulated in HDM group when the cells were intervened with LynsiRNA(P<0.05). Conclusion:Deficiencyof Lyn can increase the expression of MUC5AC in the human bronchial epithelial cells,and its mechanism may be related to regulating the STAT6 signal pathway by Lyn.关键词
Lyn/屋尘螨/人支气管上皮细胞/MUC5AC/STAT6Key words
Lyn/house dust mite/human bronchial epithelial cells/MUC5AC/STAT6分类
医药卫生引用本文复制引用
王孝芸,蓝楠,唐红梅,袁谢芳,王星,李国平..Lyn对屋尘螨诱导的人支气管上皮细胞中MUC5AC表达的影响及其机制[J].吉林大学学报（医学版）,2018,44(2):211-215,封2,6.基金项目
国家自然科学基金资助课题(81170032) （81170032）
四川省泸州市科技局科技计划项目资助课题[2016-S-67(12/23)] （12/23）
