吉林大学学报（医学版）2018，Vol.44Issue(2)：265-269,5.DOI:10.13481/j.1671-587x.20180211
MicroRNA-210对卵巢癌细胞生长及放射敏感性的影响
Effects of MicroRNA-210 on growth and radiosensitivity of ovarian cancer cells
摘要
Abstract
Objective:To investigate the role of MicroRNA-210(miR-210)in the growth process of ovarian cancer cells and its relationship with radiosensitivity,and to elucidate the effect of miR-210 on the development and treatment of ovarian cancer the possible molecular mechanism.Methods:The human ovarian cancer OVCAR3 and SKOV3 cells were cultured in vitro,miR-210 mimic and anti miR-210 inhibitors were transfected into the human ovarian cancer cell lines OVCAR3 and SKOV3,respectively,by cell transfection,and identified by Real-time PCR method;miR-210 overexpression and low expression of ovarian cancer cell models were obtained.The two kinds of cells were divided into control group,miR-210 overexpression model group and miR-210 low expression model group,and the cell proliferation activity was detected by MTT.The cells in control group and miR-210 overexpression model group were irradiated with different doses(30,50,and 100 Gy)of ionizing radiation,and the cell proliferation activity in each group was detected by MTT method.Western blotting method was used to detect the expression levels of apoptosis-related proteins in the ovarian cancer cells exposed to 50 Gy radiation dose.All the experimental cells were cultured with three double holes and repeated three times.Results:Compared with control group,the proliferation activity of the cells in miR-210 overexpression ovarian cancer cells group was enhanced,and the proliferation activity of the cells in miR-210 low expression cells group was reduced(P<0.05).After ionizing radiation,the proliferation activity of ovarian cancer cells in miRNA-210 overexpression group was enhanced compared with control group(P<0.05);the expression levels of apoptosis-related protein BAX in OVCAR3 and SKOV3 cells were significantly decreased,while the expression level of BCL-2 were significantly increased. Conclusion:miR-210 can promote the growth of ovarian cancer cells,and reduce the sensitivity of ovarian cancer cells to radiation therapy by inhibiting the apoptosis.关键词
MicroRNA-210/卵巢肿瘤/细胞凋亡/放射治疗Key words
MicroRNA-210/ovarian neoplasms/apoptosis/radiotherapy分类
医药卫生引用本文复制引用
钟莉莉,赵银龙,李炳锦,邹晓晗,程子倩,崔然吉..MicroRNA-210对卵巢癌细胞生长及放射敏感性的影响[J].吉林大学学报（医学版）,2018,44(2):265-269,5.基金项目
国家自然科学基金青年基金资助课题(81401649) （81401649）
吉林省科技厅科技发展计划项目资助课题(20180101101JC) （20180101101JC）
