吉林大学学报（医学版）2018，Vol.44Issue(2)：310-314,5.DOI:10.13481/j.1671-587x.20180219
表皮生长因子对缺氧缺糖模型大鼠骨骼肌细胞氧化损伤的保护作用
Protective effect of epithelial growth factor on oxidative damage of skeletal muscle cells in model rats of oxygen-glucose deprivation
摘要
Abstract
Objective:To set up the rat skeletal muscle L6 cell models of oxygen-glucose deprivation(OGD)in vitro,and to investigate the protective effect of EGF in deep tissue inj ury(DTI)of pressure sores.Methods:The rat skeletal muscle cells in the logarithmic phase were divided into normal control group,OGD group,5 μg·L-1 EGF+OGD group,10 μg·L-1EGF+ OGD group and 20 μg·L-1EGF+ OGD group.The survival rates of skeletal muscle cells in various groups were measured by MTT assay;the cell apoptotic rates in various groups were detected by flow cytometry;the reactive oxygen species(ROS)levels were detected by DCFH-DA;Rhodamine 123 was used to detect the mitochondrial membrane potential;the expressions of Bax and Bcl-2 proteins were determined by Western blotting method.Results:Compared with normal control group,the survival rates of skeletal muscle cells in OGD group after 24 h OGD was significantly decreased(P<0.05);the apoptotic rate was markedly increased(P<0.01);the ROS level was increased(P<0.01);the mitochondrial membrane potential was decreased(P<0.01);the ratio of Bcl-2/Bax was significantly decreased(P<0.01).Compared with OGD group, the survival rates of skeletal muscle cells in different concentrations of EGF groups were increased and the apoptotic rates were decreased,especially in 10 and 20 μg·L-1EGF groups(P<0.05 or P<0.01);the ROS levels in skeletal muscle cells in different concentrations of EGF groups were decreased and the mitochondrial membrane potential were increased,especially in 10 and 20 μg·L-1EGF groups(P<0.05 or P<0.01);the Bcl-2/Bax ratios were significantly decreased in a concentration-dependent manner,especially in 10 and 20 μg·L-1EGF groups (P<0.05 or P<0.01).Conclusion:EGF can improve the skeletal muscle cell injury induced by OGD in a concentration-dependent manner via decreasing the ROS levels and protecting the cell mitochondrial function.关键词
表皮生长因子/骨骼肌L6成肌细胞/缺氧缺糖/深部组织损伤/活性氧簇/线粒体Key words
epithelial growth factor/skeletal muscle L6 cells/oxygen-glucose deprivation/deep tissue injury/reactive oxygen species/mitochondria分类
医药卫生引用本文复制引用
许雪梅,黄笑夏,金瓯,张海邻,时洪雪..表皮生长因子对缺氧缺糖模型大鼠骨骼肌细胞氧化损伤的保护作用[J].吉林大学学报（医学版）,2018,44(2):310-314,5.基金项目
浙江省温州市科技局科技计划项目资助课题(Y20140649) （Y20140649）
浙江省科技厅自然科学基金资助课题(LY17H010005,Y14H150023) （LY17H010005,Y14H150023）
