吉林大学学报（医学版）2018，Vol.44Issue(2)：327-331,后插3,6.DOI:10.13481/j.1671-587x.20180222
葵花盘粉有效成分对小鼠高尿酸血症的治疗作用
Therapeutic effect of sunflower powder active ingredients on hyperuricemia in mice
摘要
Abstract
Objective:To investigate the therapeutic effects of small peptide,alkaloid,total flavonoids and polysaccharides extracted from sunflower plate powder on the hyperuricemia in the mice,and to clarify that the influence of sunflower powder in the uric acid(UA)level and joint swelling and its protective effect on the liver and kidney tissues.Methods:A total of 96 male Kunming rats were randomly divided into 8 groups,each group of 12 rats.One group was used as blank control group,the others were used to set up hyperuricemia models.The successful mouse models of hyperuricemia were randomly divided into model group and positive control group (allopurinol group),small molecule peptide group,alkaloi group,total flavone group,polysaccharide group and sunflower disk group.The serum UA levels were measured 7 d after administration and the liver and kidney tissues of the mice in various groups were taken.The morphological changes of liver and kidney tissues were observed by HE staining.Acute gouty arthritis model was established by injecting 3 mg sodium urate into the knee joint of the male SD rats.The experiment was divided into blank group,model group,positive control colchicine group, sunflower disc extracts(SDE)group with different concentrations(0%,20%,40%,60%,80% and 100%), 4 rats in each group.The joint circumference was measured at 0,12,24 and 48 h,respectively.The serum levels of interleukin-10(IL-10)and macrophage inflammatory protein-1α(MIP-1α)were detected by ELISA kit. Results:Compared with model group,the serum UA levels of the mice in allopurinol group,small molecule peptide group,alkaloid group,total flavonoids group and sunflower group were significantly decreased after administration (P<0.05);and the serum UA level in allopurinol group was decreased the most,followed by small molecule peptide group.The HE staining results showed that the hepatocytes in small molecule peptide group were clear, the hepatocytes had full cytoplasm,and no abnormality was observed in the kidney cells compared with blank control group.After 48 h injection of sodium urate,the degree of joint swelling was reduced by up to 27.1% in SDE group compared with model group.Compared with model group,the serum IL-10 level in 20% SDE group was significantly increased(P<0.01),and the serum MIP-1α level was also significantly increased(P<0.01). Conclusion:Sunflower powder can reduce the UA level and has protective effect on the liver and kidney tissues and it can improve the body's anti-inflammatory activity and reduce the body's inflammatory response to achieve anti-gout arthritis activity.关键词
高尿酸血症/葵花盘粉/痛风性关节炎/白细胞介素10/巨噬细胞炎性蛋白1αKey words
hyperuricemia/sunflower powder/gouty arthritis/interleukin-10/macrophage inflammatory potein-1α分类
医药卫生引用本文复制引用
戴惠咛,吕帅,王德利,李丹,张师桃,刘嘉凝,刘阳,刘小波,李婉南..葵花盘粉有效成分对小鼠高尿酸血症的治疗作用[J].吉林大学学报（医学版）,2018,44(2):327-331,后插3,6.基金项目
吉林省产业化研究项目资助课题(2017C057-3) （2017C057-3）
