重庆医学2018，Vol.47Issue(8)：1009-1011,1015,4.DOI:10.3969/j.issn.1671-8348.2018.08.001
基于荧光差异双向电泳的雷帕霉素损伤人冠状动脉内皮细胞的蛋白质组学研究
Proteomics study of rapamycin-injured human coronary artery endothelial cells based on two dimension fluorescence differential gel electrophoresis
摘要
Abstract
Objective To explore the molecular mechanisms of the effect of eluting stent drug rapamycin for injuring human coronary artery endothelial cells(HCAECs)by using the proteomics method.Methods HCAECs were treated with rapamycin,and the differentially expressed proteins were analyzed by two dimension fluorescence differential gel electrophoresis(2D-DIGE).The changed proteins were identified by MALDI-ToF-ToF.Results At least 85 differential protein spots were found,including 49 up-regulated and 36 down-regulated protein spots.Twenty-six proteins were identified by MALDI-ToF-ToF,including the endoplasmic reticulum protein,mitochondrial protein,molecular chaperones,ubiquitin system related protein,structural protein and oxidative stress related proteins,etc.Conclusion The changes of specific proteins of HCAECs injury induced by rapamycin are investigated by the proteomic method.关键词
雷帕霉素/支架洗脱药物/人冠脉内皮细胞/分子机制/蛋白质组学研究Key words
rapamycin/eluting-stent-drug/human coronary artery endothelial cells/molecular mechanisms/proteomic study分类
医药卫生引用本文复制引用
刘少军,胡坤华,钟赟..基于荧光差异双向电泳的雷帕霉素损伤人冠状动脉内皮细胞的蛋白质组学研究[J].重庆医学,2018,47(8):1009-1011,1015,4.基金项目
国家自然科学基金青年项目(81300151) （81300151）
广东省省级科技计划项目(2016A020215163). （2016A020215163）
