重庆医学2018，Vol.47Issue(8)：1019-1022,4.DOI:10.3969/j.issn.1671-8348.2018.08.004
胰岛素对大鼠胰岛β细胞VGLUT2基因表达的调控研究
Study on insulin for regulating VGLUT2 gene expression in rat pancreatic β cell
摘要
Abstract
Objective To investigate the regulation of insulin VGLUT2 gene expression in pancreatic β cells and its exactly mechanism.Methods The rat pancreatic β cell line RIN-5F was treated by different insulin concentrations and different siRNA.The changes of VGLUT2 mRNA and protein expression levels were detected by adopting real-time qPCR and Western blot.Results In-sulin with a concentration of 100,200 nmol/L significantly inhibited the expressions of VGLUT2 mRNA and protein in RIN-5RF cells(P<0.05),moreover the inhibiting effect was most significant at 100 nmol/L.After 100 nmol/L insulin treatment,the expres-sions of VGLUT2 mRNA and protein at 12,18,24 h were significantly inhibited compared with that at 0 h(P<0.05).Compared with the Blank group,Lip2000 group and Control-siRNA group,after interfering RIN-5F by using IR-siRNA,IRS1-siRNA and IRS2-siRNA,the inhibition situation of VGLUT2 mRNA and protein expressions by 100 nmol/L insulin in each group was signifi-cantly recovered(P<0.05).Conclusion Insulin at low concentration could inhibit VGLUT 2 gene expression in rat pancreatic β cell line RIN-5F.关键词
糖尿病/胰岛素/囊泡谷氨酸转运蛋白质2/受体,胰岛素/胰岛素受体底物Key words
diabetes mellitus/insulin/vesicular glutamate transport protein 2/receptor/insulin/insulin receptor substrate分类
医药卫生引用本文复制引用
王树文,李涛,林鸿程,杨丰,林荣文,陈远寿..胰岛素对大鼠胰岛β细胞VGLUT2基因表达的调控研究[J].重庆医学,2018,47(8):1019-1022,4.基金项目
国家自然科学基金面上项目(31171351) （31171351）
广东医科大学大学生创新创业训练计划项目(2011ZZDS002) （2011ZZDS002）
广东医科大学科研基金项目(M2013006). （M2013006）
