重庆医学2018，Vol.47Issue(8)：1044-1048,5.DOI:10.3969/j.issn.1671-8348.2018.08.011
ASC与Caspase-1在PBC患者外周血中的表达研究
Study on expression of ASC and Caspase-1 in peripheral blood of patients with primary biliary cirrhosis
摘要
Abstract
Objective To explore the influence of apoptosis-associated speck-like protein containing a caspase recruitment do-main(ASC)and Caspase-1 on the pathogenesis of primary biliary cirrhosis(PBC).Methods The real-time PCR,Western blot,py-rophosphate sequencing and ELISA were adopted to respectively detect the relative expressions of mRNA and protein of peripheral blood mononuclear cells(PBMS)Caspase-1 and ASC as well as the methylation status of ASC promoter region and plasma Caspase-1 and IL-18 expression levels in 30 cases of PBC and healthy controls.Results The mRNA and protein expressions of PBMC Caspase-1 and ASC in the PBC group were significantly higher than those in the control group(P<0.05).The methylation rate of ASC promoter Island1(ISI)was significantly lower than that of the control group(P<0.05),which of Island 2(IS2)was smaller than the background value and had no methylation occurrence.The levels of plasma Caspase-1 and IL-18 in the PBC group were sig-nificantly higher than those in the control group(P<0.05).The ASC mRNA in the PBC group was significantly correlated with the Caspase-1 mRNA expression(P<0.05);the methylation rates at loci 1,2,4,5 of ASC promoter region CpG island were nega-tively correlated with ASC mRNA expression(P< 0.05),and which at loci 3,6 had no correlation with their expressions(P>0.05);plasma Caspase-1 and IL-18 levels showed the obviously positive correlation.Conclusion ASC and Caspase-1 are involved in the immune inflammatory response in PBC.关键词
肝硬化,胆汁性/半胱氨酸蛋白酶类/ASC/焦磷酸测序Key words
liver cirrhosis/biliary/cysteine endopeptidases/ASC/pyrophosphate sequencing分类
医药卫生引用本文复制引用
樊佳,赵二川,叶震璇,李红梅,任智晶,张华..ASC与Caspase-1在PBC患者外周血中的表达研究[J].重庆医学,2018,47(8):1044-1048,5.基金项目
贵州省科学技术厅项目(黔科合L H 字[2014]7008号). （黔科合L H 字[2014]7008号）
