动物营养学报2018，Vol.30Issue(1)：126-137,12.DOI:10.3969/j.issn.1006-267x.2018.01.017
饲料脂肪水平对大口黑鲈形体指标、组织脂肪酸组成、血清生化指标及肝脏抗氧化性能的影响
Effects of Dietary Lipid Level on Morphology Indexes, Tissue Fatty Acid Composition, Serum Biochemical Indexes and Liver Antioxidant Indexes of Largemouth Bass(Micropterus salmoides)
摘要
Abstract
An 8-week feeding trial was conducted to evaluate the effects of dietary lipid level on morphology indexes, tissue fatty acid composition, serum biochemical indexes and liver antioxidant indexes of largemouth bass(Micropterus salmoides). Three experimental diets were prepared,in which fish oil,soybean oil and soy-bean lecithin were used as lipid sources, and the lipid levels of three experimental diets were 5.76%,11.05%and 17.65%, respectively. A total of 270 juvenile largemouth bass with an initial body weight of(4.12 ± 0.02)g were randomly divided into 3 groups with 3 replicates per group and 30 largemouth bass for each repli-cate. The results showed as follows:1)dietary lipid level had no significant effects on condition factor(CF) and hepatosomatic index(HSI)of largemouth bass(P>0.05); the intraperitoneal fat ratio(IPF)was in-creased with the dietary lipid level increasing, and it in 17.65% fat group was significantly higher than that in 5.76% lipid group(P<0.05); the viscerasomatic index(VSI)was firstly increased and then decreased with the dietary lipid level increasing, ant the 11.05% lipid group had the highest value, which was significantly higher than that in 5.76% and 17.65% lipid groups(P<0.05). 2)The proportions of polyunsaturated fatty acids(PUFA)and n-3 high unsaturated fatty acids(HUFA)in muscle of largemouth bass in 5.76% lipid group were significantly lower than those in 11.05% and 17.65% lipid groups(P<0.05),but the n-3 HUFA/n-6 HUFA was significantly higher than that in 11.05% and 17.65% lipid groups(P<0.05). The proportion of n-3 HUFA in liver of largemouth bass in 5.76% lipid group was significantly higher than that in 17.65% lipid group(P<0.05),and the n-3 HUFA/n-6 HUFA was significantly higher than that in 11.05% and 17.65% lip-id groups(P<0.05),but the proportion of n-6 HUFA was significantly lower than that in 11.05% and 17.65%lipid groups(P<0.05). 3)When dietary lipid level increased from 5.76% to 11.05% or 17.65%,the activity of alanine aminotransferase(ALT)and the contents of total cholesterol(TC), high-density lipoprotein (HDL)and low-density lipoprotein(LDL)in serum of largemouth bass were significantly increased(P<0.05), and the alanine transaminase activity was significantly decreased(P<0.05), but the differences in those indexes were not significant between 11.05% and 17.65% lipid groups(P>0.05). The triglyceride (TG)content in serum of largemouth bass in 11.05% lipid group was significantly higher than that in 5.76%and 17.65% lipid groups(P<0.05). 4)The activity superoxide dismutase(SOD), glutathione peroxidase (GSH-Px), alkaline phosphatase(AKP)and alkaline phosphatase(ALP)and the content of malondialdehyde (MDA)in liver of largemouth bass were not significant affected by dietary lipid level(P>0.05). The activity of catalase(CAT)in liver of largemouth bass in 11.05% lipid group was significantly higher than that in other two groups(P<0.05), while the activity of aspartate transaminase(AST)in liver of largemouth bass in 17.65% lipid group was significantly higher than that in other two groups(P<0.05). In conclusion, dietary lipid level can affect the lipid deposition of largemouth bass, alter the fatty acid composition in tissues, and change the ability of lipid transport and utilization by affecting the HDL and LDL contents in serum.关键词
大口黑鲈/脂肪水平/形体指标/脂肪酸组成/血清生化指标/抗氧化指标Key words
largemouth bass(Micropterus salmoides)/lipid level/morphology indexes/tissue fatty acid composition/serum biochemical indexes/antioxidant indexes分类
农业科技引用本文复制引用
朱婷婷,金敏,孙蓬,李晨晨,马红娜,罗嘉翔,袁野,周歧存..饲料脂肪水平对大口黑鲈形体指标、组织脂肪酸组成、血清生化指标及肝脏抗氧化性能的影响[J].动物营养学报,2018,30(1):126-137,12.基金项目
国家自然科学基金项目(31272670) （31272670）
宁波市农业科技攻关重大项目(2012C10025) （2012C10025）
国家科技部星火重大计划项目(2014GA701001) （2014GA701001）
浙江省重中之重一级学科(水产)开放基金 （水产）
