动物营养学报2018，Vol.30Issue(1)：180-190,11.DOI:10.3969/j.issn.1006-267x.2018.01.023
酸化剂和抗生素对黄羽肉鸡回肠黏膜菌群的影响
Effects of Acidifiers and Antibiotics on Ileal Mucosa Microbiota of Yellow-Feathered Broilers
摘要
Abstract
This experiment was conducted to study the effects of dietary long-term supplemented with acidifiers and subclinical doses of neomycin sulfate on the ileal mucosa microbiota composition and dynamic changes of yellow-feathered broilers. Eight hundred 1-day-old healthy Lingnan yellow-feathered broilers were randomly di-vided into 4 groups with 5 replicates per group and 40 broilers per replicate. Broilers in control group(group A)were fed a basal diet, and the others in groups B, C and D were fed the basal diets supplemented with 0.2% acidifier Ⅰ,0.3% acidifier Ⅱ and 50 mg/kg neomycin sulfate, respectively. The experiment lasted for 42 days. Ileal mucosa of broilers were collected and microbiota DNA were extracted at 1,7,14,21,28,35 and 42 days of age, respectively. Ileal mucosa microbiota structure of broilers were analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE),and the random clone library of 16S rD-NA of ileal mucosa microbiota of broilers at 42 days of age were established by 16S rDNA gene sequences technology. The results of dynamic change trend analysis showed that the change degree of ileal mucosa micro-biota structure of broilers in 4 groups was different, the dynamic change value at 1 to 28 days of age in 4 groups were 9% to 33%,8% to 90%,8% to 56% and 17% to 45%, respectively. The results of the random clone library of ileum mucosa microbiota analysis showed that 16 operational taxonomic units(OTUs)(form 94 sequences)were detected in group A, 7 OTUs(form 87 sequences)in group B, 5 OTUs(form 98 se-quences)in group C and 9 OTUs(form 94 sequences)in group D. The proportion of Lactobacillus in the total number of clones in groups A and D was 39.36% and 50.00%, which was significantly higher than that in groups B(6.90%)and C(10.20%)(P<0.01). The type and composition proportion of other species were different between groups A and the others. In addition,sequence related to Escherichia were not detected in all groups. These results indicate that dietary long-term supplemented with acidifiers and subclinical doses of neo-mycin sulfate can cause remarkably change of the ileal mucosa microbiota structure of Lingnan yellow-feathered broilers at 1 to 28 days of age, decreasing of the diversity of ileal mucosa microbiota of broilers at 42 days of age, change of the composition of Lactobacillus,and the changes and differences may be caused by the acidifi-er and antibiotic continuous pressure.关键词
酸化剂/硫酸新霉素/岭南黄羽肉鸡/肠道黏膜菌群/PCR-DGGE/16SrDNA克隆文库Key words
acidifier/neomycin sulfate/Lingnan yellow-feathered broilers/intestinal mucosa microbiota/PCR-DGGE/16S rDNA clone library分类
农业科技引用本文复制引用
容庭,陈庄,刘志昌,王刚,马现永,张洁,邓盾..酸化剂和抗生素对黄羽肉鸡回肠黏膜菌群的影响[J].动物营养学报,2018,30(1):180-190,11.基金项目
广东省农业科学院院长基金项目(201621) （201621）
广东省科技计划项目(2009B020307001) （2009B020307001）
