动物营养学报2018，Vol.30Issue(1)：313-320,8.DOI:10.3969/j.issn.1006-267x.2018.01.037
硫甙脱毒菌株的筛选及其发酵菜籽粕的效果
Screen of Glucosinolate Detox Strain and Its Effects on Fermentation of Rapeseed Meal
摘要
Abstract
The aim of this study was to screen the bacterial strain for detoxicating rapeseed meal and to investi-gate its effects on fermentation of rapeseed meal. Fifty-four strains with good growth were screened and isolated from mixed liquid with fermented silage and soil through cultivating in medium containing glucosinolate(GS) as a sole carbon source. Five strains were selected based on the degradation ability of GS with degradation rate greater than 30%. According to re-screening in the solid-state fermentation,strain named A9 showed high deg-radation rate(23.69%)of GS. Strain A9 has been identified as Lactobacillus farciminis after morphological observation and 16s rDNA sequence analysis. Rapeseed meal was fermented through mixing Lactobacillus far-ciminis with Bacillus subtilis and Saccharomyces cerevisiate. The results showed that the GS content of rapeseed meal fermented by mixed bacteria decreased from 37.48 to 25.96 μmol/g, and the degradation rate was 30.73%; the crude protein content increased from 38.41% to 45.44%(P<0.05); the protein structure changed, and the molecular weight of protein became smaller, the polypeptide content increased from 0.84%to 2.62%(P<0.05); the rapeseed meal fermented by mixed bacteria was yellow-brown and fluffy with a rich sour aroma, and the total acid content increased from 1.01% to 3.91%(P<0.05). In conclusion, the nutri-tional value and relish of rapeseed meal fermented by mixed bacteria can efficiently improve.关键词
硫甙/脱毒/发酵/菜籽粕/营养价值/风味Key words
glucosinolate/detoxicating/fermentation/rapeseed meal/nutritional value/relish分类
农业科技引用本文复制引用
吴正可,刘国华,蔡辉益,常文环,郑爱娟,张姝..硫甙脱毒菌株的筛选及其发酵菜籽粕的效果[J].动物营养学报,2018,30(1):313-320,8.基金项目
现代农业产业技术体系北京市家禽创新团队项目 （）
现代农业产业技术体系国家肉鸡产业技术体系项目(CARS-42) （CARS-42）
