动物营养学报2018，Vol.30Issue(1)：326-335,10.DOI:10.3969/j.issn.1006-267x.2018.01.039
干酪乳杆菌对北京黑猪保育阶段生长性能及肠道菌群的影响
Effects of Lactobacillus casei on Growth Performance and Intestinal Microbiota of Beijing Black Pigs in Nursery Stage
摘要
Abstract
This experiment was conducted to study the effects of Lactobacillus casei on growth performance and intestinal microflora of Beijing black pigs in nursery stage. A total of 120 weaned piglets for Beijing black pigs with the age of(35±2)days and average body weight of(7.53±0.21)kg were randomly distributed into 3 groups with 4 replicates per group and 10 piglets per replicate, comprising of control group, Lactobacillus casei group and chlortetracycline group. The piglets in the control group were fed a basal diet(without Lacto-bacillus casei or chlortetracycline), the piglets in the Lactobacillus casei group were fed the basal diet supple-mented with living bacteria freeze dry preparation of Lactobacillus casei,and the living bacteria count was 4.0× 109CFU/kg diet,and the piglets in the chlortetracycline group were fed the basal diet supplemented with chlor-tetracycline premix, and the chlortetracycline content was 75 mg/kg diet. The experiment lasted for 30 d. At the 28th day of the experiment,the fresh feces were collected and used for sequencing of the V3-V4 hypervari-able region of the 16S rRNA. The results showed as follows:1)compared with the control group,the average daily gain of Lactobacillus casei group and chlortetracycline group was significantly increased by 12.71%(P<0.05)and 8.58%(P<0.05), the feed/gain was decreased by 7.34%(P<0.05)and 4.52%(P>0.05), re-spectively. 2)Lactobacillus casei group increased the species richness and diversity of microbiota,as indicated by the ACE index,Chao1 index,Shannon index and Simpson index,compared with the control group and the chlortetracycline group. 3)Seventeen phyla and 206 genera were contained by three groups of feces,and 198, 200 and 197 genera were found in the control group,Lactobacillus casei group and chlortetracycline group,re-spectively. The results indicate that Lactobacillus casei supplementation in the diet has beneficial effects on the improvement of intestinal microflora structure and growth performance of Beijing black pigs in nursery stage.关键词
北京黑猪/生长性能/肠道菌群/干酪乳杆菌/金霉素Key words
Beijing black pigs/growth performance/intestinal microbiota/Lactobacillus casei/chlortetracy-cline分类
农业科技引用本文复制引用
王四新,季海峰,石国华,刘辉,王红卫,张董燕,王晶,张伟,王雅民..干酪乳杆菌对北京黑猪保育阶段生长性能及肠道菌群的影响[J].动物营养学报,2018,30(1):326-335,10.基金项目
北京市科技计划课题(Z141100002614002) （Z141100002614002）
生猪产业技术体系北京市创新团队项目(BAIC02-2017) （BAIC02-2017）
北京市农林科学院科技创新能力建设专项(KJCX20161503) （KJCX20161503）
