动物营养学报2018，Vol.30Issue(1)：368-374,7.DOI:10.3969/j.issn.1006-267x.2018.01.044
维生素E对三黄肉鸡免疫功能、组织α-生育酚沉积及脂蛋白酯酶、脂肪酸结合蛋白基因表达的影响
Effects of Vitamin E on Immune Function, α-Tocopherol Deposition and Gene Expressions of Lipoprotein Lipase and Fatty Acid-Binding Proteins in Tissues of Sanhuang Chicken
摘要
Abstract
This experiment was to investigate the effects of different dietary vitamin E levels on immune func-tion, α-tocopherol deposition and gene expressions of lipoprotein lipase(LPL)and fatty acid-binding proteins in tissues of Sanhuang chicken. A total of 256 Guangxi Sanhuang chickens at 80 days of age were randomly di-vided into 4 groups with 4 replicates per group and 16 chickens per replicate. Vitamin E at 0(control), 50, 100 and 150 mg/kg was added to a basal diet of different groups, respectively. Pre-experiment lasted for 5 d, and experiment lasted for 35 d. The results showed that compared with control group:1)the content of immu-noglobulin A(IgA)in serum was significantly increased by dietary supplementation of 50 mg/kg vitamin E (P<0.05); the content of immunoglobulin M(IgM)in serum was significantly increased by dietary supple-mentation of 50 mg/kg vitamin E(P<0.05); dietary supplementation of different levels of vitamin E had no significant effects on spleen index,and the contents of interleukin-2,tumor necrosis factor-α,interferon-γ and immunoglobulin G(IgG)in serum(P>0.05). 2)The content of α-tocopherol in liver was significantly in-creased by dietary supplementation of 50, 100 and 150 mg/kg vitamin D(P<0.05); the content of α-to-copherol in breast was significantly increased by dietary supplementation of 150 mg/kg vitamin D(P<0.05);dietary supplementation of different levels of vitamin E had no significant effects on the content of α-tocopherol in thigh(P>0.05). 3)The expression level of LPL gene in liver was significantly increased by dietary supple-mentation of 100 and 150 mg/kg vitamin D(P<0.05); the expression level of heart type-fatty acid-binding protein(H-FABP)gene in liver was significantly increased by dietary supplementation of 150 mg/kg vitamin D(P<0.05); the expression level of liver type-fatty acid-binding protein(L-FABP)gene in liver was signifi-cantly increased by dietary supplementation of 50,100 and 150 mg/kg vitamin D(P<0.05); dietary supple-mentation of different levels of vitamin E had no significant effects on the expression level of adipocyte type-fatty acid-binding protein(A-FABP)gene(P>0.05). 4)There was a significant positive correlation between dietary vitamin E level and expression levels of LPL, H-FABP and L-FABP genes in liver of Guangxi San-huang chicken(P<0.05). In summary, it is suggested that the supplementation of a high level(150 mg/kg) of vitamin E in diet can improve immune function,increase α-tocopherol deposition in tissues,and regulate ex-pression levels of LPL,H-FABP and L-FABP genes in liver of Guangxi Sanhuang chicken,which may has im-pact on the body's fat metabolism.关键词
维生素E/广西三黄肉鸡/免疫功能/α-生育酚沉积/基因表达量Key words
vitamin E/Guangxi Sanhuang chicken/immune function/α-tocopherol deposition/gene expres-sion level分类
农业科技引用本文复制引用
刘敏燕,卜泽明,刘文涛,甘书灏,穆胜龙,苏立燧,梁明振..维生素E对三黄肉鸡免疫功能、组织α-生育酚沉积及脂蛋白酯酶、脂肪酸结合蛋白基因表达的影响[J].动物营养学报,2018,30(1):368-374,7.基金项目
广西自然科学基金项目(2015GXNSFAA139061) （2015GXNSFAA139061）
科技合作项目(玉市校科合201503804) （玉市校科合201503804）
