分析测试学报2018，Vol.37Issue(2)：154-164,11.DOI:10.3969/j.issn.1004-4957.2018.02.003
液相色谱-四极杆-飞行时间质谱法快速筛查苹果与生菜中248种农药残留
Rapid Screening and Determination of 248 Pesticide Residues in Apples and Lettuces by Liquid Chromatography-Quadrupole Time-of-flight Mass Spectrometry
摘要
Abstract
A method for the simultaneous screening and determination of 248 pesticide residues in apples and lettuces was developed by liquid chromatography coupled with high resolution quadrupole time-of-flight mass spectrometry(LC-Q TOF/MS).The samples were extracted with 10 mL acetonitrile and 5 mL 0.1％ (by volume) acetic acid,cleaned up by a modified QuEChERS method.Confirmation was achieved by electro-spray ionization (ESI) in positive mode using SWATH MS acquisition,and quantification was carried out by external standard method.Two databases of accurate mass and fragment ions were created.The standard matrix-matched calibration curves of 248 target compounds were linear in the range of 0.010-0.200 mg/L,with the correlation coefficients(r) larger than 0.99.The limits of quantification for 248 target compounds all were 0.010 mg/kg.The mean recoveries for the target compounds at three spiked levels of 0.010,0.050,0.100 mg/kg ranged from 25.2％ to 128％,32.4％ to 132％ and 28.9％ to 133％,respectively with relative standard deviations of 0.98％-21.2％ (n =6).With the advantages of simplicity,timesaving,high sensitivity and good stability,the method was applicable for the simultaneous screening and determination of 248 pesticide residues in apples and lettuces,and could significantly reduce the test cost.关键词
液相色谱-四极杆飞行时间质谱/高分辨质谱/农药残留/筛查/苹果/生菜Key words
LC-Q TOF/MS/high resolution mass spectrometry/pesticide residues/screening/apple/lettuces分类
化学化工引用本文复制引用
张建莹,罗耀,宫本宁,岳振峰,赵祥龙,赵琼晖..液相色谱-四极杆-飞行时间质谱法快速筛查苹果与生菜中248种农药残留[J].分析测试学报,2018,37(2):154-164,11.基金项目
“国家质量基础的共性技术研究与应用”重点专项(NQI)(2017YFF0211103):国家质检总局项目(2017IK271) （NQI）
