分析测试学报2018，Vol.37Issue(2)：174-179,6.DOI:10.3969/j.issn.1004-4957.2018.02.005
应用可视芯片技术高通量鉴别8种鱼成分
High-throughput Identification of Eight Fish Species by Using Thin-film Biosensor Chips
摘要
Abstract
A method for the rapid and high-throughput identification of fish species using thin-film biosensor chips was developed.Based on the parvalbumin gene,a set of universal primers and specific probes were designed for eight fish species including Turbot,Silvery pomfret,Tuna,Obscura puffer,Indigo hamlet,Hairtail,Large yellow croaker and Miniatus grouper.The assay,with the characteristic of high specificity and throughput,could produce signals visualized by the unaided eye without any specific instruments.And the detection limit reached to 0.1 ng/μL.This method provided an important technical means for identifying eight fish species,which could be utilized in rapid screening for fish adulteration.关键词
可视芯片/鱼/鉴别/高通量Key words
thin-film biosensor chip/fish/identification/high-throughput分类
化学化工引用本文复制引用
韩建勋,陈颖,王斌,张九凯,马秀丽..应用可视芯片技术高通量鉴别8种鱼成分[J].分析测试学报,2018,37(2):174-179,6.基金项目
国家重点研发计划资助(2016YFD0401104) （2016YFD0401104）
