分析测试学报2018，Vol.37Issue(2)：224-230,7.DOI:10.3969/j.issn.1004-4957.2018.02.012
高效液相色谱-串联质谱法快速测定水产品中19种喹诺酮类药物残留
Determination of 19 Quinolone Antibacterials Residues in Aquatic Products by High Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
A method was developed for the determination of 19 quinolone antibacterials residues in aquatic products by high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) with PRiME HLB purification technique.In this research,the parameters such as purification,concentration,liquid phase separation and mass spectrometry were optimized.The samples were extracted with 80％ acetonitrile-water mixed solution,purified with a PRiME HLB column,and quantified by UPLC-MS/MS in MRM mode after gradient elution on an ACQUITY BEH C18 column(1.7μm,2.1 mm × 100 mm) with methanol and 5 mmol/L ammonium acetate solution containing 0.1％ formic acid as mobile phase.The results showed that there existed good linear relationships for the components in their respective concentration ranges.The average recoveries were in the range of 72.1％-119.9％ with the relative standard deviations of 2.4％-15.6％,the detection limits of 0.5 μg/kg and the quantitation limits of 1.5 μg/kg.With the advantages of good accuracy,simplicity and high sensitivity,this method is suitable for the determination of quinolone antibacterials in aquatic products,and it provides a new way for the determination of quinolone antibacterials residues in aquatic products.关键词
水产品/喹诺酮/PRiME HLB/超高效液相色谱-串联质谱Key words
aquatic products/quinolone antibacterials/PRiME HLB/high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)分类
化学化工引用本文复制引用
梁晶晶,徐潇颖,丁宇琦,陈万勤,刘柱,罗金文..高效液相色谱-串联质谱法快速测定水产品中19种喹诺酮类药物残留[J].分析测试学报,2018,37(2):224-230,7.基金项目
浙江省科技计划项目(2016C37015) （2016C37015）
浙江省食药局科研项目(SP201701) （SP201701）
